Elimination of the Neomycin Resistance Gene from the MS2 Cassette Integrated at Subtelomere 15q
6:06
Visualization of TERRA-MS2 Transcripts in Living Cells
6:49
Results: Cancer Cell Clones with MS2 Sequence Tag Created and Imaged
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Conclusion
Transcrição
This method can help answer key questions about Telomeres, such as yeast telomeric noncoding RNA TERRA localizing, and I can insist that it's telomere of origin or in trans, a different cromes-mants The main advantage of this technique is that it
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Here, we present a protocol to generate cancer cell clones containing a MS2 sequence tag at a single subtelomere. This approach, relying on the MS2-GFP system, enables visualization of the endogenous transcripts of telomeric repeat-containing RNA (TERRA) expressed from a single telomere in living cells.