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JoVE Journal

Immunology and Infection

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Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

15.3K Views

13:20 min

March 7th, 2019

March 7th, 2019

15,352 Views

0:04

Title

1:22

Reagent and Cell Preparation for ROS Measurement

5:02

Assay Preparation

5:53

Performing the Assay

11:28

Results: Flow Cytometric Measurement of ROS Production in Macrophages in Response to FcγR Cross-linking

13:00

Conclusion

Transcrição

Measurement of reactive oxygen species or ROS is notoriously problematic. In this video, we will demonstrate the reproducible measurement of ROS in response to FC-gamma receptor crosslinking using fluorescent probes and flow cytometry. The main ad

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This study demonstrates the use of flow cytometry to detect reactive oxygen species (ROS) production resulting from activation of the FcγR. This method can be used to assess changes in the antimicrobial and redox signaling function of phagocytes in response to immune complexes, opsonized microorganisms, or direct FcγR cross-linking.

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