hiPSC-CMs Purification, Small Molecule Pre-treatment, Myocardial Infarction, and Cell Transplantation
3:42
Calcium Transient and Contractility Recording
5:21
Results: Increased Engraftment of Transplanted hiPSC-CM Preconditioned with the ROCK Inhibitor Y-27632 in a Murine MI Model
7:11
Conclusion
TRANSCRIÇÃO
Our data demonstrates that transient inhibition of Rho-kinase activity enhances adhesion, survival, and engraftment of hiPSC-derived cardiomyocytes after being transplanted into the infarcted animal hearts. We provide a simple, low-cost, while hig
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In this protocol, we demonstrate and elaborate on how to use human induced pluripotent stem cells for cardiomyocyte differentiation and purification, and further, on how to improve its transplantation efficiency with Rho-associated protein kinase inhibitor pretreatment in a mouse myocardial infarction model.