Salivary Gland Section Irradiation and Antibody Staining
5:17
Results: Representative Characteristics of In Vivo Salivary Gland Section 2D Cultures
6:53
Conclusion
TRANSCRIÇÃO
This protocol allows the evaluation of cell to cell interactions between diverse cell types within a tissue, and ex vivo molecular and pharmacological manipulations that would be less feasible in vivo. The main advantage of this technique is the e
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Using three-dimensional organotypic cultures to visualize morphology and functional markers of salivary glands may provide novel insights into the mechanisms of tissue damage following radiation. Described here is a protocol to section, culture, irradiate, stain, and image 50–90 μm thick salivary gland sections prior to and following exposure to ionizing radiation.