Galactose-Induced Motor Protein Growth, Expression, and Harvesting
1:39
Cell Lysis and Soluble Protein Extraction
2:36
IgG Affinity Purification
3:17
DNA Oligonucleotide Labeling and TEV Cleavage
4:50
Chassis Purification
5:56
Results: Representative Motor Ensemble Production
7:26
Conclusion
Transcrição
Our protocol is designed to probe the biophysical mechanisms governing the transport of cargo by teams of identical or oppositely directed cytoskeletal motor proteins. By using DNA origami for molecular construction, this method can choose for the
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The goal of this protocol is to form ensembles of molecular motors on DNA origami nanostructures and observe the ensemble motility using total internal reflection fluorescence microscopy.