Reprogramming of Dermal Fibroblasts to iPSCs (Induced Pluripotent Stem Cells)
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Results: Skin Fibroblast
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Conclusion
Transcrição
The following protocol provides instructions to generate autologous human-induced pluripotent stem cells through the fast and efficient reprogramming of human dermal fibroblasts isolated from a skin punch biopsy. The procedures can be carried out
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Cell reprogramming requires the introduction of key genes, which regulate and maintain the pluripotent cell state. The protocol described enables the formation of induced pluripotent stem cells (iPSCs) colonies from human dermal fibroblasts without viral/integrating methods but using non-modified RNAs (NM-RNAs) combined with immune evasion factors reducing cellular defense mechanisms.