CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

0:58

Preparation of CRISPR Gene Editing and Guide RNA Design to Generate miRNA Cluster Knockout Cell Lines

2:06

Generation of Stable Lentiviral Cell Lines Carrying the DOX-Inducible Cas9 Expression Cassette

3:20

Performing CRISPR Reactions by Cas9 Induction and Synthetic Guide RNA Transfection of Cells Using a High-Throughput Format

7:22

PCR Genotyping of CRISPR Cell Lines Using Crude Cell Lysates

8:37

Results: High-Throughput CRISPR Experimental Design to Generate Multiple miR-888 Cluster Deletion Combinations

9:51

Conclusion