Results: Measuring the Conformational Dynamics of aSyn
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Transcrição
All organisms have disorder in their proteome, estimated up to 33%These regions are observable by hydrogen/deuterium exchange mass spectrometry, but only in the millisecond time range. Our protocol will show you how to do this successfully. This i
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The structural ensemble of monomeric alpha-synuclein affects its physiological function and physicochemical properties. The present protocol describes how to perform millisecond hydrogen/deuterium-exchange mass spectrometry and subsequent data analyses to determine conformational information on the monomer of this intrinsically disordered protein under physiological conditions.