A Method for Extracting Pigments from Squid Doryteuthis pealeii

Summary

A protocol for the extraction of pigments from the nanostructured granules in squid Doryteuthis pealeii chromatophores is presented.

Abstract

Cephalopods can undergo rapid and adaptive changes in dermal coloration for sensing, communication, defense, and reproduction purposes. These capabilities are supported in part by the areal expansion and retraction of pigmented organs known as chromatophores. While it is known that the chromatophores contain a tethered network of pigmented granules, their structure-function properties have not been fully detailed. We describe a method for isolating the nanostructured granules in squid Doryteuthis pealeii chromatophores and demonstrate how their associated pigments can be extracted in acidic solvents. To accomplish this, the chromatophore containing dermal layer is first manually isolated using a superficial dissection, and the pigment granules are removed using sonication, centrifugation, and washing cycles. Pigments confined within the purified granules are then extracted via acidic methanol solutions, leaving nanostructures with smaller diameters that are void of visible color. This extraction procedure produces a 58% yield of soluble pigments isolated from granules. Using this method, the composition of the chromatophore pigments can be determined and used to provide insight into the mechanism of adaptive coloration in cephalopods.

Introduction

Cephalopods such as squid, cuttlefish, and octopus have the ability to dynamically alter their appearance for camouflaging and signaling.^1-6 This capability is supported in part by the selective areal expansion of pigmented organs known as chromatophores.^4,7-9 Chromatophores are soft actuators that contain a network of nanostructured pigment granules confined within a cytoelastic sacculus that is anchored radially by muscle fibers.^1,3 As they are actuated, chromatophores expand by 500% in presented surface area distributing the granules throughout the organ.^3,7,10,11 When this action is concerted over a number of chromatophores, the overall coloration of the animal is changed. While it is known that the pigment granules contribute to this color change, their composition remains unknown. We describe a procedure to isolate and purify chromatophore pigments which may be adapted for future compositional studies.

The isolation of pigment granules involves a multistep extraction, homogenization, and purification procedure.^3,12 Chromatophore containing tissue is harvested through careful extirpation from the cephalopod. A digestion and homogenization process is then used to dissociate the surrounding tissue and separate the chromatophore cells. The nanostructured granules are then isolated and purified from the remaining chromatophores using repeated sonication and centrifugation. Upon purification, pigments are extracted from the granules in a process that is adapted from the extraction of visible color from butterfly wings using acidic methanol solutions.¹³ Scanning electron microscopy (SEM) and spectrophotometry are used to confirm that the chromatophore pigments are successfully extracted using this process.

This method describes the isolation of chromatophore granules which is used to explore the molecular contributions to coloration in cephalopods.¹² Small molecule extractions from whole animals can often be a long and tedious process. The aim here is to inform future researchers of an effective and facile protocol for the acquisition of pigments from the nanostructured granules in cephalopods.

Protocol

Invertebrate animal studies conducted herein are not regulated in the United States; therefore the Institutional Animal Care and Use Committee has no authority for review of such protocols. In lieu of these not falling under the jurisdiction of regulation in the United States, the authors hereby state that these studies were conducted with sincere effort towards the ethical use, care, and treatment of these animals, the number of individuals was minimized and these efforts are consistent with the Basel Declaration and the International Council for Laboratory Animal Science (ICLAS) ethics guidelines.

1. Doryteuthis pealeii (D. pealeii) Dissection

1. Purchase decapitated squid D. pealeii from the Marine Resources Department at the Marine Biological Laboratory at Woods Hole, MA or from any fresh-fish market. Do not use specimens that have been filleted or previously altered in order to apply this procedure. Specifically, the chromatophore layer must still be intact on the specimen.
   1. If the animals are not used immediately, store them at -20 °C until use. To defrost the specimens, place them in room temperature water for 1 hr prior to dissection.
2. Using stainless steel straight fine-point dissection scissors, cut along the posterior side of the animal. Begin at the base of the ventral mantle, and end at the fin region.
3. Remove all of the internal organs (stomach, gill, ink gland, reproductive organs, systemic heart, and brachial heart) by lifting them with dissecting forceps and severing their connective tissue using dissection scissors. Discard organs in a specimen bag.
4. Use dissecting T-pins to pin the mantle (fin side up) in a standard 11-1/2" x 7-1/2" x 1-1/2" aluminum dissection pan containing a flexible dissection pad. Submerge the tissue in 250 ml of seawater that has been filtered through a disposable polystyrene vacuum filtration system containing 0.2 µm pore sizes.
5. Carefully remove the epidermal skin layer (opaque color) with dissecting forceps, keeping the chromatophore containing skin layer intact. Once the epidermal layer is removed, discard it in a specimen bag.
6. Dissect small (1 cm x 1 cm) sections of the chromatophore layer with forceps and dissection scissors. Collect samples in 1.5 ml micro-centrifuge tubes (fill one-half of each empty tube with the dissected tissue).
7. Add 500 µl of filtered seawater to the tissue containing tubes. The samples can be stored overnight at 4 °C.

2. Isolating Chromatophore Pigment Granules

1. Tissue Digestion
   1. Preparation of Papain/collagenase
      1. Weigh out 30 mg of collagenase and 5 mg of papain using a 4" x 4" low nitrogen weigh paper. Transfer these into a 50 ml polypropylene centrifuge tube with a screw cap.
      2. Measure 10 ml of deionized water using a graduated pipette. Add directly to vial containing papain/collagenase. Vortex on the highest setting until the solution is clear.
   2. Removing Extracellular Tissue
      1. Centrifuge the collected chromatophore tissue from step 1.7 for 5 min at 14,000 x g. Discard the top layer of sea water into a plastic waste bucket.
      2. Add 500 µl of the papain/collagenase solution to the tissue using a P1000 variable volume single channel micropipette. Vortex each sample for 1-2 min on the highest setting.
      3. Sonicate the samples for 5 min at a frequency of 40 kHz to dissociate cells from the surrounding tissue.
      4. Centrifuge for 5 min at 14,000 x g. Discard the supernatant into a plastic waste bucket.
      5. Centrifuge the collected chromatophore tissue for 5 min at 14,000 x g. Discard the top layer of sea water into a plastic waste bucket. Repeat once again.
      6. Manually remove any remaining large tissue sections that did not digest in this process with forceps before proceeding to the next step.
2. Isolating the Pigment Granules
   1. Preparation of Homogenization Buffer
      1. Weigh 2.38 g of (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES, 100 mM), 0.095 g of magnesium chloride (10 mM), 0.856 g of potassium-aspartate (50 mM), and 0.015 g of dithiothreitol (1 mM). Transfer into a 150 ml polystyrene container with a screw cap. Add one commercial mini tablet protease inhibitor.
      2. Measure 100 ml of deionized water using a graduated cylinder. Add directly to the container containing the homogenization salts. Vortex until the solution is clear.
   2. Homogenization of Pigment Granules
      1. Centrifuge each sample from step 2.1 at 14,000 x g for 5 min. Discard the supernatant into a plastic waste bucket. This sample should be void of extracellular tissue.
      2. Add 500 µl of homogenization buffer to each tube and vortex for 1-2 min each to thoroughly mix the samples. Sonicate the samples for 30 min (~40 kHz) and follow with 5 min of centrifugation at 14,000 x g.
      3. Discard the supernatant into a plastic waste bucket, and repeat the previous step 2-3 times to ensure the complete digestion of the chromatophore sacculus and protein tethers. The remaining solution should contain a light yellow supernatant and a red-colored pellet containing the isolated pigment granules.

3. Pigment Extraction

1. Preparation of Acidic Methanol (HCl-MeOH)
   1. Carefully add 25 µl of concentrated (36.5-38 % (w/w)) hydrochloric acid (HCl) to 5 ml of methanol (MeOH). Store the HCl-MeOH solution in a screw cap glass sample vial. Swirl gently to uniformly mix the solution.
      NOTE: The more acidic the solution, the more pigment will be extracted during the first extraction.
2. Extraction of Pigment
   1. Centrifuge the pigment granule suspension (from 2.2.2) for 5 min at 14,000 x g and discard the supernatant into a plastic waste bucket.
   2. Add 500 µl of the HCl-MeOH solution and vortex each sample for ~3-4 min. Sonicate the samples for 10 min (~40 kHz), then centrifuge for 5 min at 14,000 x g.
   3. Using a transfer pipette, collect the supernatant in a 1 dram screw-top glass vial. The supernatant should be dark red in color.
   4. Repeat the extraction step 3.2.2 until no further color is extracted (~4-5 washes).
      NOTE: Each extraction will yield ~1.5 ml of pigment. The remaining colorless pellet is the pigment extracted granules. NOTE: Both colorless granules and extracted pigments can be stored in water at 4 °C until further use.

4. Characterization throughout Extraction Process

1. Scanning Electron Microscopy
   1. Image the granules (unreacted from step 2.2.1 and pigment-extracted from step 3.2.3) using scanning electron microscopy (SEM) to verify the purity of the extraction process.
   2. To prepare granule samples for imaging, first centrifuge both unreacted granules and pigment extracted granules for 5 min at 14,000 x g, and discard the supernatant into a plastic waste bucket. Re-suspend these in 1 ml of ethanol, and deposit 2-3 drops of the granule suspension onto aluminum SEM stubs using a transfer pipette.
   3. After drying the samples overnight, sputter coat with gold-platinum coating for 3 min to achieve a 15 nm coating.
   4. Image using a SEM with a Schottky emitter and a secondary electron detector. Use a 5-7 kV beam voltage and a working distance between 8 and 9 mm to image these materials.
2. Ultraviolet-visible (UV-vis) Spectrophotometric Measurements
   1. Monitor the absorbance profiles for all 3 conditions (unreacted granule from 2.2.2, extracted pigment from 3.2.3, and pigment extracted granules from 3.2.4) using a dual beam spectrophotometer from 200-800 nm.
   2. Collect a blank measurement using deionized water in a 1 ml cuvette for the unreacted granules and the pigment extracted granules. Collect UV-Vis absorption spectra for both samples.
   3. Collect a blank measurement using the acidic methanol solution in a 1 ml cuvette for the extracted pigment. Collect UV-Vis absorption spectrum for sample.
   4. Determine maximum wavelength of each sample by identifying the wavelength at which the absorbance peak reaches its maximum relative height.

Results

Chromatophores are dissected from the D. pealeii dorsal mantle (Figure 1A, 1B). Once they are removed, chromatophores are lysed and purified using centrifugation and washing cycles to isolate out the pigmented granules (Figures 2A, 2B). Acidic methanol solutions (HCl-MeOH) are used to extract the pigment from the granules (Figure 2C), yielding a soluble pigment extract and insoluble, colorless pi...

Discussion

We have demonstrated a method to extract pigments from squid chromatophore granules. By specifically targeting the granules, our goal is to determine their role in mediating adaptive coloration. This method differs from previous reports designed to characterize cephalopod pigments using bulk tissue samples¹⁴ or freeze-dried skin¹⁵.

While this protocol is effective at extracting chromatophore pigments, it is limited to small reaction scales. Dissecting one squid yields ~11...

Materials

Name	Company	Catalog Number	Comments
Collagenase	Alfa Aesar	9001-12-1	No hazard
Dithiothreitol	Sigma-Aldrich	3482-12-3	Irritant, acute toxicity
HEPES	Sigma-Aldrich	7365-45-9	Mild irritant
Hydrochloric acid	EMD Chemicals	7647-01-0	Corrosive
k-Aspartate	Sigma-Aldrich	1115-63-5	Reacts violently with oxidants
Magnesium Chloride	Sigma-Aldrich	7786-30-3	Mild eye irritant
Methanol	Pharmco-AAPER	67-56-1	Highly flammable
Mini tablet prtoease inhibitor	Sigma-Aldrich	469315-90-01	Corrosive to metal and skin
Papain	Sigma-Aldrich	9001-73-4	Irritant