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Abstract

Behavior

The Three-Chamber Choice Behavioral Task using Zebrafish as a Model System

Published: April 14th, 2021

DOI:

10.3791/61934

1Department of Biology, American University, 2Center for Behavioral Neuroscience, American University, 3Department of Chemistry, American University, 4Department of Neuroscience, American University

Abstract

Neurodegenerative diseases are age-dependent, debilitating, and incurable. Recent reports have also correlated hyperglycemia with changes in memory and/or cognitive impairment. We have modified and developed a three-chamber choice cognitive task similar to that used with rodents for use with hyperglycemic zebrafish. The testing chamber consists of a centrally located starting chamber and two choice compartments on either side, with a shoal of conspecifics used as the reward. We provide data showing that once acquired, zebrafish remember the task at least 8 weeks later. Our data indicate that zebrafish respond robustly to this reward, and we have identified cognitive deficits in hyperglycemic fish after 4 weeks of treatment. This behavioral assay may also be applicable to other studies related to cognition and memory.

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Keywords Three chamber Choice Task

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