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Abstract

Medicine

Murine Orchiectomy and Ovariectomy to Reduce Sex Hormone Production

Published: November 17th, 2023

DOI:

10.3791/64379

1Department of Ophthalmology, UT Southwestern Medical Center, 2Department of Molecular Biology, UT Southwestern Medical Center, 3Hamon Center for Regenerative Science and Medicine, UT Southwestern Medical Center, 4Peter O'Donnell Jr. Brain Institute, UT Southwestern Medical Center

Abstract

Sex hormone signaling plays a critical role in multiple organ systems as well as in the progression of various diseases, including neurodegenerative disease. The manipulation of sex hormone levels in the murine model system allows for the study of their impact on organs/tissues and within disease progression. Orchiectomy - the surgical removal of the testes - and ovariectomy - the surgical removal of the ovaries - provide a method to deplete the endogenous sex hormones so that the precise hormone levels can be provided through drug or other delivery methods. Here, we provide rapid and minimally invasive methods for both orchiectomy and ovariectomy in the murine model system for the reduction of sex hormones. This protocol details the surgical preparation and excision of the testes through the scrotal sac, and excision of the ovaries via two incisions in the right and left lateral dorsum.

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