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Delivering Intrathecal Injections to Neonatal Mice

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02:28 min

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March 8th, 2024

DOI :

10.3791/201022-v

March 8th, 2024

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1Department of Genetics, Yale University School of Medicine, 2Department of Neuroscience, Yale University School of Medicine, 3Department of Pediatrics, Yale University School of Medicine

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Keywords Intrathecal Injection
Neonatal Mice
Spinal Canal
Intervertebral Space
25 Or 10 Microliter Syringe
Anesthesia
Midline Palpation
Needle Insertion Angle
Injection Volume
Post injection Recovery
Fast Green Dye
YFP Reporter Mice
CRISPR Cas9 Gene Editing

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A Comprehensive Protocol for Delivering Intrathecal Injections in Neonatal Mice
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