Yazarlar
Bize Ulaşın

Oturum Aç

Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs

38 Views

•

02:01 min

•

March 1st, 2024

DOI :

10.3791/201479-v

March 1st, 2024

•
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, 2Hollings Cancer Center, Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research, National Cancer Institute-Frederick

Transkript

Daha Fazla Video Keşfet

Transfection
293FT Cells
PCMV5 NanoLuc CRAF
PCMV5 14 3 3 Halo
Trypsin EDTA
DMEM
Cell Suspension
Nanoluciferase
Expression Constructs
Transfection Media
Transfection Reagent
Microcentrifuge
Halo Tagged Proteins

Seriden

CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
JoVE Logo

Gizlilik

Kullanım Şartları

İlkeler

Bize Ulaşın

KÜTÜPHANEYE TAVSİYE ET

JoVE HABER BÜLTENLERİ

Araştırma

Eğitim

Yazarlar

Kütüphaneciler

JoVE Hakkında

Telif Hakkı © 2020 MyJove Corporation. Tüm hakları saklıdır