NIH HL-10342 tarafından desteklenir.

1. Prosedür için hazırlanıyor Bir ilk edinilir ve aşağıdaki öğeleri hazırlamak gerekir: <ol><li> Kanül. 20-35 g farelerin entübasyon için, biz uzun bir 1 ya da 1.5 inç, 20 gauge IV kateter (BD Insylte, Sparks, MD veya Jelco Optiva, Carlsbad, CA) kullanın. Yeni bir steril sonda her bir fare için kullanılabilir, fakat kateterler, bir gecede% 70 etanol içinde ıslatarak sterilizasyon sonrasında yeniden kullanılabilir. Fare yutak veya trakea ne steril eldivenler ve araçların kullanımı da dahil olmak üzere steril uygun temizlik prosedürleri, olmasına rağmen, takip edilmelidir. </li><li> Fiberoptik kablo. Edmund Optik 0,5 mm&#39;lik optik kablo ≈ 70 cm kullanır, ancak uzunluk kritik değildir. Bir jilet ile uzunlukta kablo kestikten sonra, kenar nispeten keskin sol yana fiber kenarından düzeltti olduğundan emin olmak önemlidir ve trakeal duvar delmek için çok çaba almaz. Bununla birlikte, bu ilar çok kolay 1000 grit zımpara kağıdı bir parça (bir video ve Şekil 1&#39;de gösteri görmek dokunmadan ucu kenarları ucundan yaklaşık 2 cm lif tutarak ve sonra birkaç saniye için küçük daireler çizerek bu kenar yumuşatmak için MacDonald, ve diğ. 10). Diğer ucu bir kauçuk durdurucu sokulur. Bu, en kolay şekilde, ilk önce iğnenin geri çekilmesi, durdurucu vasıtasıyla bir 18-gauge iğne itme iğne deliği vasıtasıyla optik fiber ekleme ile başarılır. Kauçuk tıpa (örneğin, NCL-150, Volpi USA, ya da başka herhangi bir ışık kaynağı ya da hatta daha az bir 150 watt) yaklaşık 150 watt halojen ışık kaynağı ile bağlantılıdır. Bu sıcak ışık kaynağına çok yakın bulunduğu zaman sıradan lastik veya mantar yakabilir yana, silikon kauçuk (veya diğer ısıya dayanıklı malzemeden) yapılmış bir stoper kullandığınızdan emin olmak için önemlidir. </li></ol> 2. Entübasyon Sahne <ol><li> Şekil 1 ve 2&#39;ye bakınız. Fiber optik kablo t takınhrough silikon kauçuk tüp kısa bir parça (≈ 0.8 mm ID x 4 mm D.Ç., Cole-Palmer, EW-96410-13). Hala fiber optik kablo ayarlanabilir izin verirken, oldukça sıkı bu lastik tüp bağla. Kanülün Luer sonunda rahatça silikon tüp takılması kanül içine fiber optik kablo konumunu giderir. Bu kanül uç önünde kanül ≈ 4 mm kadar uzanır, böylece fiber optik kablonun pozisyonunu ayarlar. </li><li> Üst dişleri (Şekil 3) ile asılı duran bir dikey destek üzerinde anestezi uygulanmış fare, koyun. Çoğu araştırmacılar kendilerini bakan fare ventral tarafı ile iyi görselleştirme bulabilirsiniz. Çok hafifçe dilini dışarı çekin ve başparmak ve işaret parmağı ile tutun. Orta parmak boyun ve plastik destek arasına yerleştirilir. Işaret parmağı ve başparmak ile dil üzerinde çekiş gücü ağzı açmak için, ve entübasyon yol düzeltmek için kullanılır, kafa ve boyun arkasından açı orta parmak ile ayarlanırŞekil 3 &#39;de gösterilmiştir. </li><li> Bir ışık kaynağı ve introduser olarak fiber optik kablo kullanarak görüntülenmiştir ses telleri ile itin. Kabloları görünmüyorsa, hafifçe destek olarak orta parmak ile dil üzerinde sert çekin. Takıldığında, daha kanül ≈ 5 mm ilerlemek. Sonra, çok dikkatli olmak, kanül hareket fiber optik kablo çekmek değil. Fare Uzan ve kanül sabitlemek bant parçası ve Plasticine (modelleme kil) bir parçası üzerinde kanül hub desteklemez, Şekil 4&#39;te gösterilmektedir. </li><li> Yalnız bir işlem olduğundan 3. adımda prosedürü kolay öğretilebilir ve hatta, ortaya olamaz. Ancak, dil ve başının arkasında destek çekiş ince ayarlamalar ile neredeyse bu deneyin herkese yakında ses telleri görselleştirmek için fare konumlandırmak için doğru yolu bulmak. </li></ol>

<ol>
	<li>Brown, R. H., Walters, D. M., Greenberg, R. S., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+method+of+endotracheal+intubation+and+pulmonary+functional+assessment+for+repeated+studies+in+mice.">A method of endotracheal intubation and pulmonary functional assessment for repeated studies in mice.</a> J. Appl. Physiol. 87, 2362-2365 (1999).</li><li>Walters, D. M., Wills-Karp, M., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+cellular+profile+and+lung+function+with+repeated+bronchoalveolar+lavage+in+individual+mice.">Assessment of cellular profile and lung function with repeated bronchoalveolar lavage in individual mice.</a> Physiol. Genomics. 2, 29-36 (2000).</li><li>Rivera, B., Miller, S., Brown, E., Price, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+method+for+endotracheal+intubation+of+mice+and+rats+used+in+imaging+studies.">A novel method for endotracheal intubation of mice and rats used in imaging studies.</a> Contemporary Topics in Laboratory Animal Science / American Association for Laboratory Animal Science. 44, 52-55 (2005).</li><li>Hamacher, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microscopic+wire+guide-based+orotracheal+mouse+intubation:+description,+evaluation+and+comparison+with+transillumination.">Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination.</a> Laboratory Animals. 42, 222-230 (2008).</li><li>Spoelstra, E. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+and+simple+method+for+endotracheal+intubation+of+mice.">A novel and simple method for endotracheal intubation of mice.</a> Laboratory Animals. 41, 128-135 (2007).</li><li>Zhao, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+technique+for+retrograde+intubation+in+mice.">A technique for retrograde intubation in mice.</a> Lab Animal. 35, 39-42 (2006).</li><li>Vergari, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+method+of+orotracheal+intubation+in+mice.">A new method of orotracheal intubation in mice.</a> European Review for Medical and Pharmacological Sciences. 8, 103-106 (2004).</li><li>Vergari, A., Polito, A., Musumeci, M., Palazzesi, S., Marano, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Video-assisted+orotracheal+intubation+in+mice.">Video-assisted orotracheal intubation in mice.</a> Laboratory Animals. 37, 204-206 (2003).</li><li>Hastings, R. H., Summers-Torres, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+Laryngoscopy+in+Mice.">Direct Laryngoscopy in Mice.</a> Contemporary Topics in Laboratory Animal Science / American Association for Laboratory Animal Science. 38, 33-35 (1999).</li><li>MacDonald, K. D., Chang, H. Y., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+improved+simple+method+of+mouse+lung+intubation.">An improved simple method of mouse lung intubation.</a> J. Appl. Physiol. 106, 984-987 (2009).</li><li>Ewart, S. L., Levitt, R. C., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Respiratory+system+mechanics+in+mice+measured+by+end-inflation+occlusion.">Respiratory system mechanics in mice measured by end-inflation occlusion.</a> Journal of Applied Physiology. 79, 560-566 (1995).</li><li>MacDonald, K. D., McKenzie, K. R., Mitzner, W., Zeitlin, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lung+Mechanics+in+Heterozygous+CF+Mice+after+Repeated+LPS+Dosing.">Lung Mechanics in Heterozygous CF Mice after Repeated LPS Dosing.</a> Am. J. Respir. Crit. Care Med. 175 (4), A930 (2007).</li></ol>

Yazarların hiçbiri ifşa herhangi bir çıkar çatışması var.

Burada anlatılan prosedür birçok avantajı vardır. İlk cihaz basit ve nispeten ucuz .. Aparat imalatı hiçbir özel alet ya da pahalı ekipman gerektirmez. Bir kateter kullanımı da ışık kaynağının tek bir uygulayıcıya trakeal açıklığa yaklaştıkça trakea açıklığı görme kaybeder asla anlamına gelir olduğunu tanıtır. Bir 0.5 mm uygulayıcıya kullanımı da daha büyük bir kanül bir ilk ekleme ile meydana gelebilecek travmayı en aza indirmek için sunulmaktadır. Biz benzer bir optik pro...

1999 yılında, Brown ve ark. Fare akciğer 1 intübasyon için bir yöntem tarif eden bir kağıt yayınlanmıştır. Böyle bir teknik uzunlamasına çalışmalarda 2 bireysel farelerde tekrar solunum fonksiyon veya bronkoalveoler lavaj yaparken önemli bir yararı da vardır. O orijinal kağıt beri, fare entübasyon 3-9 farklı yaklaşımlar tarif ettiğim birkaç diğer kağıtlar olmuştur. Bu yöntemlerin hepsi başarıyla kullanılabileceği gibi, genellikle önemli eğitim veya maliyet gerektirir. Böyle entübasyon ile ana konulardan biri entübasyon kanülü yaklaşımlar olarak trakea bekleyen ekleme yaklaşıyor olduğunu, kanülün kendisi ışık ve dolayısıyla gitmek gerekir nerede görselleştirme engeller. Böylece, ekleme en önemli zamanda kör hale gelir. Bu yazıda basit ve masrafsız böylece nispeten az eğitim veya başarılı entübasyon sağlamak, bu görselleştirme sorunu ortadan kaldırmak için nasıl gösterirdeneyim.

<table border="1"><tbody><tr><td> Malzeme </td><td> Şirket </td><td> # Katalog </td><td> Yorumlamak </td></tr><tr><td> Entübasyon kanülü </td><td> BD Insylte, Sparks, MD veya Jelco Optiva, Carlsbad, CA </td><td></td><td> 1-inç uzunluğunda, 20 gauge intravenöz (IV) kateter </td></tr><tr><td> Fiber-optik kablo </td><td> Edmund Optik, Barrington, NJ </td><td> # NT02-542 </td><td> Yaklaşık 2-ft 0.5 mm optik fiber uzunluğu (Haberleşme dereceli plastik fiber). Trakea içine yerleştirilen fiber ucunun kenarı hafifçe 1.000 zımpara Emory kağıt üzerinde birkaç saniye için ucu sürüklerken ≈ 2 cm ucundan lif tutarak ve sonra küçük daireler çizerek yuvarlatılmış olmalıdır. </td></tr><tr><td> Işık kaynağı </td><td> Volpi </td><td> NCL-150 </td><td> Biz bile çok daha düşük watt ile 150-W halojen ışık kaynağı, herhangi bir eşdeğer ışık kaynağı kullanımı olsa da,kullanılabilir. </td></tr><tr><td> Silikon kauçuk tıpa </td><td></td><td></td><td> A # 1 silikon kauçuk tıpa Bu tasvirde kullanılan ışık kaynağı uyuyor. Farklı ışık kaynakları, farklı bir boyut gerektirebilir. </td></tr><tr><td> Küçük silikon kauçuk tüp </td><td> Cole-Palmer </td><td> EW-96410-13 </td><td> Silikon kauçuk borudan yapılmış bir ≈ 1.5 cm&#39;lik bir parça (0,8 iç çapı 4 mm dış çapı) </td></tr><tr><td> Açılı sopalı </td><td></td><td></td><td> Bizim pleksiglas dan constucted, ancak bir iplik ya da tel bir hemen hemen dik açılı olarak fare tutmak için üzerine yerleştirilebilir için herhangi bir malzeme de kullanılabilir. </td></tr></tbody></table>

a simple method of mouse lung intubation

Akciğer fonksiyon ölçümleri için fareler Entübe bir basit bir prosedür sınırlı sayıda ya da pahalı hayvan ile uzunlamasına çalışmalarda çeşitli avantajları olurdu. Bu daha çok rutin olarak yapılan olmadığını nedenlerinden biri var bunu başarmak için yollarını tanımlamak birçok yayınlanmış çalışmaları olmasına rağmen, nispeten zor olmasıdır. Bu yazıda bu entübasyon ile ilişkili en önemli engellerden biri, entübasyon tüm süre boyunca trakea görselleştirme olduğunu ortadan kaldıran bir yordam göstermek. Yaklaşım fare trakea içine entübasyon kanül doğrudan bir uygulayıcıya olarak hizmet veren bir 0,5 mm fiberoptik ışık kaynağı kullanır. Biz bunu en az birkaç hafta süre boyunca bireysel farelerde akciğer mekaniği ölçmek için bu yordamı kullanmanız mümkün olduğunu göstermektedir. Tekniği nispeten daha az masraf ve uzmanlığı ile ayarlanabilir, ve rutin nispeten az eğitim ile gerçekleştirilebilir. Bu mak gerekirmümkün herhangi bir laboratuvar rutin böylece böylece gerekli farelerin sayısını en aza indirir ve kendi kontrolü olarak her fare kullanılarak istatistiksel gücünün artırılması, bireysel farelerde uzunlamasına çalışmalar sağlayan bu entübasyon yürütmek için e.

Yöntemin bir değerlendirme olarak, 27.7, ortalama ağırlığı (ortalama ± SEM) ± 0.40 g ile 20 dört haftalık erkek BALB / c fareleri kullanılmıştır. 11. Daha önce tarif edildiği gibi akciğer direncini bir sistem kullanılarak ölçüldü olduğunda da birbirini takip eden beş hafta üzerinde çalışıldı. Her fare IP enjeksiyon yoluyla ketamin (100 mg / g BW) ve salin ksilazin (15 mikrogram / g BW) ile anestezi yapıldı. Yukarıda bahsedildiği gibi sonra entübe edildi. Kanül trakea ve de...

Watch this Scientific Journal Video about A Simple Method of Mouse Lung Intubation at JoVE.com

A Simple Method of Mouse Lung Intubation

Bu yazıda akciğer fonksiyonu ölçümleri veya fareler kurtarmak için ve daha sonraki dönemlerde de incelenebilir sağlar pulmoner instilasyon için fare entübasyon bir striaghforward ve etkili bir yöntem açıklanır. Işlem doğrudan soluk borusu yanması pahalı olmayan bir fiber optik ışık kaynağı içerir.

Fare Akciğer Entübasyonun Basit Bir Yöntem

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

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Medicine

50.5K Görüntüleme.  Johns Hopkins Bloomberg School of Public Health. Bu yazıda akciğer fonksiyonu ölçümleri veya fareler kurtarmak için ve daha sonraki dönemlerde de incelenebilir sağlar pulmoner instilasyon için fare entübasyon bir striaghforward ve etkili bir yöntem açıklanır. Işlem doğrudan soluk borusu yanması pahalı olmayan bir fiber optik ışık kaynağı içerir.

Video: A Simple Method of Mouse Lung Intubation

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

Despite the well-known close association, direct evidence linking disturbed flow to atherogenesis has been lacking. We have recently used a modified version of carotid partial ligation methods [1,2] to show that it acutely induces low and oscillatory flow conditions, two key characteristics of disturbed flow, in the mouse common carotid artery. Using this model, we have provided direct evidence that disturbed flow indeed leads to rapid and robust atherosclerosis development in Apolipoprotein E knockout mouse [3]. We also developed a method of endothelial RNA preparation with high purity from the mouse carotid intima [3]. Using this mouse model and method, we found that partial ligation causes endothelial dysfunction in a week, followed by robust and rapid atheroma formation in two weeks in a hyperlipidemic mouse model along with features of complex lesion formation such as intraplaque neovascularization by four weeks. This rapid in vivo model and the endothelial RNA preparation method could be used to determine molecular mechanisms underlying flow-dependent regulation of vascular biology and diseases. Also, it could be used to test various therapeutic interventions targeting endothelial dysfunction and atherosclerosis in considerably reduced study duration.

This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.

Kısmi ligasyon Fare Karotis Arter Disturbed Akış Kaynaklı Ateroskleroz ve Karotis Endotel RNA İzolasyonu Basit Bir Yöntem Bir Model

Despite the well-known close association, direct evidence linking disturbed flow to atherogenesis has been lacking. We have recently used a modified ...

Tıp

Guidelines for Elective Pediatric Fiberoptic Intubation

Fiberoptic intubation in pediatric patients is often required especially in difficult airways of syndromic patients i.e. Pierre Robin Syndrome. Small babies will desaturate very quickly if ventilation is interrupted mainly to high metabolic rate. We describe guidelines to perform a safe fiberoptic intubation while maintaining spontaneous breathing throughout the procedure. Steps requiring the use of propofol pump, fentanyl, glycopyrrolate, red rubber catheter, metal insuflation hook, afrin, lubricant and lidocaine spray are shown.

We describe guidelines to perform a safe and efficient elective fiberoptic intubation in pediatric patients while maintaining spontaneous ventilation.

Seçmeli Pediatrik Fiberoptik Entübasyon için rehber

Fiberoptic intubation in pediatric patients is often required especially in difficult airways of syndromic patients i.e. Pierre Robin Syndrome. Small ...

A Simple Guide Screw Method for Intracranial Xenograft Studies in Mice

The grafting of human tumor cells into the brain of immunosuppressed mice is an established method for the study of brain cancers including glioblastoma (glioma) and medulloblastoma. The widely used stereotactic approach only allows for the injection of a single animal at a time, is labor intensive and requires highly specialized equipment. The guide screw method, initially developed by Lal et al.,1 was developed to eliminate cumbersome stereotactic procedures. We now describe a modified guide screw approach that is rapid and exceptionally safe; both of which are critical ethical considerations. Notably, our procedure now incorporates an infusion pump that allows up to 10 animals to be simultaneously injected with tumor cells.
To demonstrate the utility of this procedure, we established human U87MG glioma cells as intracranial xenografts in mice, which were then treated with AMG102; a fully human antibody directed to HGF/scatter factor currently undergoing clinical evaluation2-5. Systemic injection of AMG102 significantly prolonged the survival of all mice with intracranial U87MG xenografts and resulted in a number of complete cures. 
This study demonstrates that the guide screw method is an inexpensive, highly reproducible approach for establishing intracranial xenografts. Furthermore, it provides a relevant physiological model for validating novel therapeutic strategies for the treatment of brain cancers.

In order to evaluate novel therapeutic paradigms for the treatment of glioma, physiological relevant models are essential. We utilize an implantable guide screw procedure for establishment of intracranial xenograft models that is more rapid and safer than stereotactic approaches.

Basit Fare İntrakranial Xenograftlarında Çalışmaları Kılavuzu Vida Yöntemi

The grafting of human tumor cells into the brain of immunosuppressed mice is an established method for the study of brain cancers including glioblastoma ...

Noninvasive Intratracheal Intubation to Study the Pathology and Physiology of Mouse Lung

The use of a model that mimics the condition of lung diseases in humans is critical for studying the pathophysiology and/or etiology of a particular disease and for developing therapeutic intervention. With the increasing availability of knockout and transgenic derivatives, together with a vast amount of genetic information, mice provide one of the best models to study the molecular mechanisms underlying the pathology and physiology of lung diseases. Inhalation, intranasal instillation, intratracheal instillation, and intratracheal intubation are the most widely used techniques by a number of investigators to administer materials of interest to mouse lungs. There are pros and cons for each technique depending on the goals of a study. Here a noninvasive intratracheal intubation method that can directly deliver exogenous materials to mouse lungs is presented. This technique was applied to administer bleomycin to mouse lungs as a model to study pulmonary fibrosis.

The use of a model that mimics the condition of lung diseases in humans is critical for studying the pathophysiology and/or etiology of a particular disease and for developing therapeutic intervention. Here a noninvasive intratracheal intubation method that can directly deliver exogenous materials to mouse lungs is presented.

Noninvaziv Entübasyon Fare Akciğer Patolojisi ve Fizyolojisi Eğitim için

The use of a model that mimics the condition of lung diseases in humans is critical for studying the pathophysiology and/or etiology of a particular ...

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Recent significant advances in stem cell research and bioengineering techniques have made great progress in utilizing biomaterials to regenerate and repair damage in simple tissues in the orthopedic and periodontal fields. However, attempts to regenerate the structures and functions of more complex three-dimensional (3D) organs such as lungs have not been very successful because the biological processes of organ regeneration have not been well explored. It is becoming clear that angiogenesis, the formation of new blood vessels, plays key roles in organ regeneration. Newly formed vasculatures not only deliver oxygen, nutrients and various cell components that are required for organ regeneration but also provide instructive signals to the regenerating local tissues. Therefore, to successfully regenerate lungs in an adult, it is necessary to recapitulate the lung-specific microenvironments in which angiogenesis drives regeneration of local lung tissues. Although conventional in vivo angiogenesis assays, such as subcutaneous implantation of extracellular matrix (ECM)-rich hydrogels (e.g., fibrin or collagen gels or Matrigel - ECM protein mixture secreted by Engelbreth-Holm-Swarm mouse sarcoma cells), are extensively utilized to explore the general mechanisms of angiogenesis, lung-specific angiogenesis has not been well characterized because methods for orthotopic implantation of biomaterials in the lung have not been well established. The goal of this protocol is to introduce a unique method to implant fibrin gel on the lung surface of living adult mouse, allowing for the successful recapitulation of host lung-derived angiogenesis inside the gel. This approach enables researchers to explore the mechanisms by which the lung-specific microenvironment controls angiogenesis and alveolar regeneration in both normal and pathological conditions. Since implanted biomaterials release and supply physical and chemical signals to adjacent lung tissues, implantation of these biomaterials on diseased lung can potentially normalize the adjacent diseased tissues, enabling researchers to develop new therapeutic approaches for various types of lung diseases.

Recapitulation of the organ-specific microenvironment, which stimulates local angiogenesis, is indispensable for successful regeneration of damaged tissues. This report demonstrates a novel method to implant fibrin gels on the lung surface of living mouse in order to explore how the lung-specific microenvironment modulates angiogenesis and alveolar regeneration in adult mouse.

Fare Akciğer Fibrin Gel implantasyonu Akciğer özel Anjiogenez Eğitim için

Recent significant advances in stem cell research and bioengineering techniques have made great progress in utilizing biomaterials to regenerate and ...

Intubation-mediated Intratracheal (IMIT) Instillation: A Noninvasive, Lung-specific Delivery System

Respiratory disease studies typically involve the use of murine models as surrogate systems. However, there are significant physiologic differences between the murine and human respiratory systems, especially in their upper respiratory tracts (URT). In some models, these differences in the murine nasal cavity can have a significant impact on disease progression and presentation in the lower respiratory tract (LRT) when using intranasal instillation techniques, potentially limiting the usefulness of the mouse model to study these diseases. For these reasons, it would be advantageous to develop a technique to instill bacteria directly into the mouse lungs in order to study LRT disease in the absence of involvement of the URT. We have termed this lung specific delivery technique intubation-mediated intratracheal (IMIT) instillation. This noninvasive technique minimizes the potential for instillation into the bloodstream, which can occur during more invasive traditional surgical intratracheal infection approaches, and limits the possibility of incidental digestive tract delivery. IMIT is a two-step process in which mice are first intubated, with an intermediate step to ensure correct catheter placement into the trachea, followed by insertion of a blunt needle into the catheter to mediate direct delivery of bacteria into the lung. This approach facilitates a &#62;98% efficacy of delivery into the lungs with excellent distribution of reagent throughout the lung. Thus, IMIT represents a novel approach to study LRT disease and therapeutic delivery directly into the lung, improving upon the ability to use mice as surrogates to study human respiratory disease. Furthermore, the accuracy and reproducibility of this delivery system also makes it amenable to Good Laboratory Practice Standards (GLPS), as well as delivery of a wide range of reagents which require high efficiency delivery to the lung.

Intubation-mediated Intratracheal IMIT Instillation: A Noninvasive, Lung-specific Delivery System

Intubation-mediated intratracheal (IMIT) instillation of reagents is an excellent, noninvasive method for studying respiratory disease, as well as a method for instilling therapeutic reagents directly into the lung. It is a rapid and highly reproducible method which is suitable for preclinical testing.

Entübasyon-aracılı intratrakeal (IMIT) damlatma: A Noninvaziv, Lung-özel Dağıtım Sistemi

Respiratory disease studies typically involve the use of murine models as surrogate systems. However, there are significant physiologic differences ...

Mouse Pneumonectomy Model of Compensatory Lung Growth

In humans, disrupted repair and remodeling of injured lung contributes to a host of acute and chronic lung disorders which may ultimately lead to disability or death. Injury-based animal models of lung repair and regeneration are limited by injury-specific responses making it difficult to differentiate changes related to the injury response and injury resolution from changes related to lung repair and lung regeneration. However, use of animal models to identify these repair and regeneration signaling pathways is critical to the development of new therapies aimed at improving pulmonary function following lung injury. The mouse pneumonectomy model utilizes compensatory lung growth to isolate those repair and regeneration signals in order to more clearly define mechanisms of alveolar re-septation. Here, we describe our technique for performing mouse pneumonectomy and sham pneumonectomy. This technique may be utilized in conjunction with lineage tracing or other transgenic mouse models to define molecular and cellular mechanism of lung repair and regeneration.

Mouse pneumonectomy is a commonly employed model of compensatory lung growth. This procedure can be used in conjunction with lineage tracing or transgenic mouse models to elucidate underlying mechanisms.

Telafi Akciğer Büyüme Fare Pnömonektomi Modeli

In humans, disrupted repair and remodeling of injured lung contributes to a host of acute and chronic lung disorders which may ultimately lead to ...

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. As this technology expands and improves, the ability to potentially evaluate and improve the quality of substandard lungs prior to transplant is a critical need. In order to more rigorously evaluate these approaches, a reproducible animal model needs to be established that would allow for testing of improved techniques and management of the donated lungs as well as to the lung-transplant recipient. In addition, an EVLP animal model of associated pathologies, e.g., ventilation induced lung injury (VILI), would provide a novel method to evaluate treatments for these pathologies. Here, we describe the development of a rat EVLP lung program and refinements to this method that allow for a reproducible model for future expansion. We also describe the application of this EVLP system to model VILI in rat lungs. The goal is to provide the research community with key information and &#8220;pearls of wisdom&#8221;/techniques that arose from trial and error and are critical to establishing an EVLP system that is robust and reproducible.

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.

İzole Yöntemi<em&gt; Ex vivo</emBir Rat Modelinde&gt; Akciğer Perfüzyon: Sıçan EVLP Programı Geliştirme Öğrenilmiş Dersler

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed ...

A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine

Preparing whole mounts of the mouse small intestine and colon for subsequent analysis or quantification can be time consuming and difficult. We describe the use of a simple device to cut and &#8216;roll&#8217; mouse intestines to rapidly prepare whole mount preparations of superior and uniform quality to that which can be achieved by hand. The device comprises a base that holds 4 stainless steel rods and a top, which acts a cutting guide. The rods are inserted into the lumen of the small intestine [divided into thirds] and the colon. The rods and samples are then placed over a piece of filter paper or card into the holding slots in the base of the device. The top of the device is then positioned and serves as a cutting guide. The two angled sections in the center of the top piece are used to guide a knife or scalpel and cut the intestines longitudinally on the top of the rods. Once the intestinal sections have been cut, the top is removed and the card,&#160;tissue and rods gently removed from the device and placed on the bench. The rods are then gently rolled sideways to flatten and stick the intestinal segments onto the underlying piece of filter paper or card. The final preparation can then be examined or fixed and stored for later analysis. The preparations are invaluable for the study of intestinal changes in normal or genetically modified mouse models. The preparations have been used for the study and quantification of the effects of inflammation (colitis), damage, pre-cancerous lesions (aberrant crypt foci (ACFs) and mucin depleted foci (MDFs)) and polyps or tumors.

The use of a simple device to cut and &#8216;roll&#8217; mouse intestines to rapidly prepare whole mount preparations is described.

Basit Aygıt Hızla Fare Bağırsak Tüm Mounts Hazırlanır

Preparing whole mounts of the mouse small intestine and colon for subsequent analysis or quantification can be time consuming and difficult. We describe ...

An Improved Method for Rapid Intubation of the Trachea in Mice

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity is a commonly used model to study both acute lung injury and fibrosis, and multiple methods have been developed for administering bleomycin (and other toxic agents) into the lungs. However, many of these approaches, such as transtracheal instillation, have inherent drawbacks, including the need for strong anesthetics and survival surgery. This paper reports a quick, reproducible method of intratracheal intubation that involves mild inhaled anesthesia, visualization of the trachea, and the use of a surrogate spirometer to confirm exposure. As a proof of concept, 8-12 week old C57BL/6 mice were administered either 2.0 U/kg of bleomycin or an equivalent volume of PBS, and both damage and fibrotic endpoints were measured post-exposure. This procedure allows researchers to treat a large cohort of mice in a relatively short period with little expense and minimal post-procedure care.

This article presents a rapid and simple method for administering bleomycin directly into the mouse trachea via intubation. Key advantages of this method are that it is highly reproducible, easy to master, and does not require specialized equipment or lengthy recovery times.

Farelerde Trakea hızlı intübasyon için geliştirilmiş bir yöntem

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity ...