Isolation of Sialic Acids from Milk and from Liver Tissue
3:26
Preparation of a Mixed Sialic Acid Standard
4:26
Fluorescence Derivatization of Sialic Acids
5:18
HPLC Analysis of Sialic Acid Derivatives
6:15
Results: Quantification of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice
7:05
Conclusion
Transkript
The overall goal of this methodology is the detection of N-Acetylneuraminic acid and N-Glycolylneuraminic acid in the liver and milk of wild-type and CMAH knock-out mice using HPLC analysis. This method can help to answer questions in the field of
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We describe a HPLC-based method for the determination of N-acetylneuraminic acid and N-glycolylenuraminic acid in mouse liver and milk.