Fixation, Paraffin Embedding, and Liver Block Sectioning
2:35
Immunohistochemistry for Wide-spectrum Cytokeratin (wsCK) and αSMA
4:08
Imaging and Quantification of Bile Ducts (BDs)
5:27
Results: Determining BD to PV Ratio
7:00
Conclusion
Transkript
This protocol enables the investigator to quantify bile duct development in mouse models of liver disease. It also allow for evaluating the effects of genetic modifiers on bile duct development. The advantage of this technique is that it is a stra
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We present a rather simple and sensitive method for accurate quantification of bile duct density in the mouse liver. This method can aid in determining the effects of genetic and environmental modifiers and the effectiveness of potential therapies in mouse models of biliary diseases.