Monoclonal antibodies are known as mono-specific antibodies which are produced from a single B-lymphocyte clone and the monovalent antibodies which bind to the same epitope. Recently, the monoclonal antibody-based ELISA has become our emerging pla
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This article provides a detailed protocol for the preparation and evaluation of monoclonal antibodies against natural products for use in various immunoassays. This procedure includes immunization, cell fusion, indirect competitive ELISA for positive clone screening, and monoclonal hybridoma preparation. The specifications for antibody characterization using MALDI-TOF-MS and ELISA analyses are also provided.