This method can help answer key questions in the blood-testis barrier of biology fields about gene candidates, viruses, or environmental toxins that may affect blood-testis barrier function or integrity. Three days before the surgery, treat at lea
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Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.