Inducing PCD (protocatechuate 3,4 dioxygenase) Expression in E. coli
3:39
Nickel Affinity Chromatography Purification of PCD
4:46
Nuclease Activity Assay
6:19
PCA (Protocatechuic Acid) Oxidation and Nuclease Activity Assays
8:01
Results: Purification of PCD
9:23
Conclusion
Transkript
Our protocol yields highly active PCD free from detectable nuclease, which can be used in any solution where aqueous oxygen is a contaminant. The techniques we use are easy to replicate and ensure that no nuclease contaminants are present in the p
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Protocatechuate 3,4-dioxygenase (PCD) can enzymatically remove free diatomic oxygen from an aqueous system using its substrate protocatechuic acid (PCA). This protocol describes the expression, purification, and activity analysis of this oxygen scavenging enzyme.