Platelets should be free from blood cells and proteins when studying their biochemical and physiological properties. Therefore, we have developed a protocol to purify platelets from mouse blood. This technique can purify platelets using iohexol gr
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Here, mouse blood was collected in the presence of an anti-coagulant. The platelets were purified by iohexol gradient medium using low speed centrifugation. The platelets were activated with thrombin to investigate if they were viable. The quality of the purified platelets was analyzed by flow cytometry and microscopy.