Surgery for Adeno‐Associated Virus (AAV) Injection into the Brown Adipose Tissue (BAT) in Mice
2:41
Results: Identifying the Potential Flaws Leading to Unsuccessful Injection
3:18
Conclusion
Transkript
This protocol generates brown fat-specific knockout mice using a Cre-LoxP, CRISPR-Cas9, and adeno-associated virus single-guide RNA system. It includes a surgical technique to safely administer AAV into the brown fat. The main advantage of this te
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In this protocol, we describe the technical procedures to generate brown adipose tissue (BAT)-specific knockout mice leveraging a combined Cre-LoxP, CRISPR-Cas9, and adeno-associated virus (AAV) single-guide RNA (sgRNA) system. The described steps include the design of the sgRNAs, the preparation of the AAV-sgRNA particles, and the microinjection of AAV into the BAT lobes.