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DOI :
10.3791/4110-v
•
7:44 min
August 26th, 2012
Chapters
0:05
Title
1:27
High-throughput Growth of Bacterial Cultures
2:38
Robotic Purification of Recombinant Proteins
4:06
Protein Purification
6:32
Representative Protein Purification Results
7:08
Conclusion
我々は、液体処理ロボットを用いた組換えタンパク質のアフィニティータグ精製する方法を説明します。この方法は、一般にハイスループットフォーマットで可溶性Hisタグ融合タンパク質の小スケールの精製にも適用可能である。
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