October 23rd, 2013
•We describe a methodology to perform genetic analysis in Chlamydia based on chemical mutagenesis and whole genome sequencing. In addition, a system for DNA exchange within infected cells is described that can be used for genetic mapping. This method may be broadly applicable to microbial systems lacking transformation systems and molecular genetic tools.
Tags
Vídeos Relacionados
Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados