This work was supported by an NRSA predoctoral grant 1F31DC012985 (to R.D.) and by NIH grants to J.C.
We would like to thank Dr. Linnea Weiss for helpful comments on the manuscript, Dr. Ryan Joseph for help compiling figures, and Dr. Frederic Marion-Poll for helpful technical advice. We would also like to acknowledge the helpful comments of four reviewers.

The following protocol complies with all the animal care guidelines of Yale University.
1. Reagents and Equipment Preparation
<ol>
	<li>Recording equipment setup (Figure 1A).
	<ol>
		<li fo:keep-together.within-page="always">Choose a room for rig setup that is free of large variations in temperature or humidity and also isolated from sources of electrical and mechanical noise, such as refrigerators and centrifuges. 
		<img alt="Figure 1" fo:content-width="5in" fo:src="/files/ftp_upload/51355/51355fig1highres.jpg" src="/files/ftp_upload/51355/51355fig1.jpg" /> 
		Figure 1.&#160;(A) Overview of recording rig setup. Stereomicroscope (a) is mounted on anti-vibration platform (b). Reference electrode holder (c) is mounted on platform opposite the headstage (d), via micromanipulators. An outlet plastic tube (e) delivering humidified air stream directed at the fly preparation is also mounted on the platform. The headstage is connected to the amplifier (f), which is connected to the digital acquisition system (DAS) (g), which is connected to a PC (h). (B) Configuration of electrodes and outlet tube: reference electrode on the left, recording electrode on the right, and air stream outlet tube directed at fly preparation. <a href="https://www.jove.com/files/ftp_upload/51355/51355fig1highres.jpg" target="_blank">Click here to view larger image.</a></li>
		<li>Mount stereomicroscope to center of anti-vibration table or platform.</li>
		<li>Attach micromanipulators for the reference electrode/insect preparation and headstage/recording electrode to the left and right of the microscope, respectively, using magnetic stands.</li>
		<li>Mount outlet plastic tube in a third micromanipulator to the rear of the microscope, oriented such that the tube opening is pointed toward location of fly prep (see Figure 1B).</li>
		<li>Using flexible plastic tubing, attach outlet plastic tube to a vacuum flask partially filled with water. Connect a small aquarium pump to bubble air through the water in the flask, generating a humidified air stream through the outlet plastic tube towards the fly.</li>
		<li>Mount fiber optic light source off the vibration table, orienting the outputs to illuminate the preparation by reflecting light via a piece of white card paper directly below the preparation. Ensure that the light source does not rest on the table. Note: the benefit of reflecting the light source on a paper disc is two-fold: it improves the contrast, making sensilla easier to visualize, and it prevents heating of the preparation that would result from direct light.</li>
		<li>Plug tastePROBE amplifier into the digital acquisition system (DAS), and the DAS into a personal computer, according to supplier manual. Plug foot pedal trigger in and arrange under workspace. Note: Electrically isolated wall sockets for the amplifier and DAS are highly desirable.</li>
		<li>Electrically ground microscope, micromanipulators, and light source by connecting metal components to table using alligator clips and lengths of insulated electrical wire and electrical tape. Electrically ground metal platform by connecting to building ground or DAS, which is grounded through power supply plug.</li>
		<li>Install appropriate acquisition software for the DAS of choice on the personal computer. Note: Ensure that the digital acquisition drivers are compatible with the operating system on the PC.</li>
		<li>Configure software amplification (10-100x), signal filtering (typically a Bessel bandpass filter set from 100 Hz-3,000 Hz), and sampling rate (at least 10 KHz). Note: Signal amplitudes from gustatory neurons are typically in the 0.5-2 mV range, so the display scale is set to facilitate their visualization. Note: The 100 Hz filter helps to exclude extraneous electrical noise; however, it changes the shape of spikes and can make advanced spike sorting more challenging. Alternatively, a 1 Hz filter can be used.</li>
		<li>Optionally, a Faraday cage can be set up around the whole vibration table. However, small sheets of aluminum foil are usually sufficient to reduce any noise generated by the external environment or investigator.</li>
	</ol>
	</li>
	<li fo:keep-together.within-page="always">Glass Electrode Preparation 
	<img alt="Figure 2" fo:content-width="5in" fo:src="/files/ftp_upload/51355/51355fig2highres.jpg" src="/files/ftp_upload/51355/51355fig2.jpg" /> 
	Figure 2.&#160;Reference and recording electrodes. Photograph under magnification of glass capillaries pulled into reference electrode, with (A) and without (B) tip broken, and recording electrode (C). White bar represents 2 mm. <a href="https://www.jove.com/files/ftp_upload/51355/51355fig2highres.jpg" target="_blank">Click here to view larger image.</a>
	<ol>
		<li>Pull the reference electrode from a glass capillary using a pipette puller instrument. Note: The exact settings of the pipette puller program will vary from instrument to instrument. Try to achieve a very long gradual taper. The pore size at the tip is not crucial because the tip will be broken before fly preparation (Figures 2A and 2B); however, make sure that the diameter of the tapered length of the electrode is neither too thin, which will not allow for sufficient immobilization of the labellum, nor too large, which could damage the gustatory neurons or rupture the salivary glands.</li>
		<li>Pull recording electrode from a borosilicate glass capillary with filament using a pipette puller instrument. Try to achieve a taper that is shallower than that of the reference electrode, and a pore diameter of approximately 10-15 &#956;m (Figure 2C)28.</li>
	</ol>
	</li>
	<li>Tastant solutions preparation
	<ol>
		<li>Use Beadle-Ephrussi Ringer solution (B&#38;E) as the reference electrode electrolyte. To make one liter of B&#38;E, dissolve 7.5 g NaCl, 0.35 g KCl, and 0.279 g CaCl2&#8729;2H2O in one liter of ultrapure water. Store smaller aliquots at -20 &#176;C.</li>
		<li>Use 30 mM tricholine citrate solution (TCC) as the recording electrode electrolyte and solvent for tastant solutions25, if bitter or sugar GRN responses are to be measured. Alternatively, 1-3 mM potassium chloride solution can be used if responses of the water cell are to be measured.</li>
		<li>To make tastant solutions, weigh appropriate amount of tastant in powder form and add to TCC to make an initial stock concentration. Use this to make serial dilutions from this initial stock to yield the desired concentration for testing. Note: If tastants do not readily dissolve in water, another solvent, such as ethanol, can be used to make initial stock concentration. An appropriate control solution of TCC and solvent without tastant should be used in this case.</li>
		<li>Store aliquots long term at -20 &#176;C. Store one working aliquot of a tastant solution at 4 &#176;C for recording use for up to a week, depending on chemical properties of tastant.</li>
	</ol>
	</li>
</ol>
2. Drosophila&#160;Preparation
<img alt="Figure 3" fo:content-width="5in" fo:src="/files/ftp_upload/51355/51355fig3highres.jpg" src="/files/ftp_upload/51355/51355fig3.jpg" /> 
Figure 3.&#160;Preparation of fly for recording. (A) Insertion position of reference electrode into dorsal thorax of fly. The white arrow indicates the reference electrode. (B) Intermediate position of reference electrode: advanced through neck and head, proboscis not yet extended. (C,D) Fly with reference electrode in final position with tip of electrode inside labellum, and proboscis fully extended. <a href="https://www.jove.com/files/ftp_upload/51355/51355fig3highres.jpg" target="_blank">Click here to view larger image.</a>
<ol>
	<li>Collect newly eclosed flies for recording from well-maintained fly cultures, grown under temperature- and humidity-controlled conditions, and age them 5-10 days in fresh culture vials before recording.</li>
	<li>Chill microscope plate on ice for 15-30 min before preparing fly.</li>
	<li>Backfill glass reference electrode with B&#38;E solution using a long, thin plastic needle of 0.5 mm diameter, such as a spinal needle and 1&#160;ml syringe and gently tap out any bubbles. Break small amount of tip off using forceps and use capillary action to draw out all remaining bubbles with a tissue, observing under dissecting microscope.</li>
	<li>Slide B&#38;E-filled reference electrode onto wire of reference electrode holder, taking care not to introduce air bubbles.</li>
	<li>Aspirate fly into a P200 pipette tip, using fly aspirator built from tubing, mesh, and pipette tip;29 place in ice bucket and chill for 30-60 sec.</li>
	<li>Remove microscope plate from ice, wipe off any moisture, and position underneath microscope. Gently tap fly out of pipette tip onto microscope plate. 
	Note: the fly should be sufficiently immobilized to manipulate easily.</li>
	<li>Under low magnification, gently remove the forelegs with one pair of forceps, while holding the thorax stable with the other pair of forceps. Position the fly on its ventral side, dorsal side facing up. Note: Always be careful to avoid touching the labellum with the forceps at all times during the preparation process to minimize mechanical damage.</li>
	<li>While holding the fly in place with one pair of forceps, insert the reference electrode at the midline of the posterior dorsal thorax. A suggested angle of entry is approximately forty-five degrees, in the direction of the head (Figure 3A).</li>
	<li>Secure the reference electrode holder with modeling clay such that the fly is visible underneath the microscope at high magnification. Maneuver and angle the glass electrode through the neck and head, by sliding the fly towards the reference electrode holder using two pairs of forceps. Note: Work quickly but smoothly; it is easier to complete this step while the fly is still immobilized from the cold (Figure 3B).</li>
	<li>Gently extend the proboscis with one pair of forceps, while sliding the fly further down the glass reference electrode, until the tip of the electrode is inside the labellum and the proboscis is fully extended (Figures 3C and 3D). Note: Take care not to puncture any part of the proboscis tissue or distend the edge of the labellum with the reference electrode, as this may damage the fly and/or taste neurons and affect the recording quality.</li>
</ol>
3. Recording from Labellar Sensilla
<img alt="Figure 4" fo:content-width="5in" fo:src="/files/ftp_upload/51355/51355fig4highres.jpg" src="/files/ftp_upload/51355/51355fig4.jpg" /> 
Figure 4.&#160;Recording from fly. (A) labellum of fly preparation on left with recording electrode aligned for contact on right, under high magnification. (B) recording electrode and single sensillum on labellum in contact, under high magnification. <a href="https://www.jove.com/files/ftp_upload/51355/51355fig4highres.jpg" target="_blank">Click here to view larger image.</a>
<ol>
	<li>Always ground yourself by touching the metal surface of the anti-vibration table or platform prior to touching any equipment during recording process! Note: It is extremely important not to deliver a static charge to the headstage as that can damage the circuitry.</li>
	<li>Secure reference electrode holder to micromanipulator mounted on air table of recording rig. Position one lobe of labellum in microscope field of view, under high magnification (typically at least 140X), and in line with humidified air stream.</li>
	<li>Turn on humidified air stream, computer, DAS, and amplifier. Open acquisition software.</li>
	<li>Rinse and fill glass recording electrode with desired tastant.
	<ol>
		<li>Rinse glass recording electrode with ultrapure water by using a syringe and plastic tubing28 to pull small amounts of water through the tube at least ten times.</li>
		<li>Rinse recording electrode with tastant at least five times. Fill recording electrode approximately one-third to halfway full with tastant and remove from tubing. If there are air bubbles, tap to release or simply refill the electrode.</li>
		<li>Slide electrode onto silver wire of the headstage quickly and smoothly so as not to introduce air bubbles.</li>
	</ol>
	</li>
	<li>Stimulate single sensillum with tastant-filled recording electrode.
	<ol>
		<li>Use the micromanipulator to bring the recording electrode aligned with sensillum of interest.</li>
		<li>Press foot pedal to trigger acquisition mode of the amplifier.</li>
		<li>Advance the recording electrode with the fine control knob of micromanipulator carefully until it makes contact with tip of sensillum and recording commences.</li>
		<li>Remove electrode after 1-2 sec.</li>
		<li>Repeat step 3.5 with other sensilla, if desired. Note: Wait at least 1 min in between presentations to the same sensillum. If recording with a single tastant for a prolonged period of time, the tastant solution may dry out and the solution in the tip may become more concentrated. This can be remedied by gently contacting the tip of the glass electrode with smooth paper to remove a small amount of liquid by capillary action.</li>
	</ol>
	</li>
	<li>To record responses to another tastant, rinse and load recording electrode with new tastant and repeat step 3.4. Note: Thoroughly rinsing the electrode between tastants is absolutely crucial to avoid cross-contamination.</li>
	<li>Save data files periodically with identifying information, such as date, genotype, and tastants. Note: It is important to keep a written record of the tastant and sensillum identity of each presentation during recording session for data analysis.</li>
</ol>

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R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Software+filter+for+detecting+spikes+superimposed+on+a+fluctuating+baseline.">Software filter for detecting spikes superimposed on a fluctuating baseline.</a> Journal of neuroscience. 37, 1-6 (1991).</li><li>Meunier, N., Marion-Poll, F., Lansky, P., Rospars, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estimation+of+the+individual+firing+frequencies+of+two+neurons+recorded+with+a+single+electrode.">Estimation of the individual firing frequencies of two neurons recorded with a single electrode.</a> Chem Senses. 28, 671-679 (2003).</li><li>Meunier, N., Marion-Poll, F., Rospars, J. P., Tanimura, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Peripheral+coding+of+bitter+taste+in+Drosophila.">Peripheral coding of bitter taste in Drosophila.</a> Journal of neurobiology. 56, 139-152 (2003).</li></ol>

The authors have nothing to disclose.

Labellar sensilla vary in the ease of recording due to differences in morphology and anatomical organization. Sometimes a sensillum does not respond to any tastants, even one that is known to elicit a positive response. The frequency with which this occurs varies depending on sensillum type. L sensilla are most consistently responsive and are relatively easy to access due to their length. In general, S sensilla are consistently responsive, but their short length and position on the labellum make good contact challenging....

The sense of taste allows an insect to detect a vast range of soluble chemicals and plays an important role in the acceptance of a nutritious substance, or the rejection of a noxious or toxic one. Taste is also thought to play a role in mate selection, through the detection of pheromones1-5. These important and diverse functions have made the insect taste system a compelling target of investigation into how sensory systems translate environmental cues into relevant behavioral outputs.
The primary unit of the Drosophila melanogaster taste system is the taste hair, or sensillum. Molecules enter the sensillum via a pore at its tip2,6. Sensilla are found on the labellum, the legs, the wing margin, and the pharynx6.&#160;On the labellum, the number and location of sensilla is stereotyped. There are three morphological classes of sensilla based on length: the long (L), intermediate (I), and short (S) sensilla7,8. Each sensillum contains either two (I-type) or four (L- and S- type) gustatory receptor neurons (GRNs)9. Different GRNs respond to different categories of taste stimuli: bitter, sugar, salt and osmolarity7,10 and express different subsets of gustatory receptors8,11-13. Only I and S-type sensilla contain bitter-responsive GRNs8,10. The GRNs project to the subesophageal ganglion (SOG) and their activation by taste molecules is relayed to the higher central nervous system for decoding, resulting in a behavioral response6. The relatively small number of neurons and the amenability to molecular and behavioral analysis make the Drosophila taste system an excellent model for the investigation of gustatory systems in general. The relative ease with which the system can be manipulated via genetic mutation or the GAL4-UAS expression system also serves as a valuable tool14,15.
Because these sensilla protrude from the surface of the labellum, they make excellent targets for electrophysiology. The firing of the GRNs can be monitored using extracellular recording. Historically, the side-wall recording method, which uses a glass electrode inserted into the sensillum to record neuronal activity,26 has been used. However, this method is technically challenging to perform, and it is difficult to record for long from each preparation. The tip-recording method, which measures the response of the neurons with an electrode that simultaneously delivers a tastant, has since become the method of choice9,16. It has been utilized to investigate the taste system of Drosophila melanogaster8,10,17,18 as well as a number of other insect species19-23. It has been greatly facilitated by the development of the tastePROBE amplifier, which overcame one of the major drawbacks of the tip-recording method by compensating for the large potential difference between the reference electrode and the insect sensillum, allowing the GRN action potentials to be recorded without excessive amplification or filtering24. Another important development was the use of tricholine citrate as the recording electrolyte25. TCC suppresses responses from the osmolarity-sensitive GRN and does not stimulate the salt-sensitive GRN, making responses generated by bitter and sugar tastants much easier to analyze25.
Here we describe how tip recording of Drosophila labellar sensilla is currently performed in the Carlson laboratory. This protocol will explain how to establish a suitable electrophysiology rig, how to prepare the fly, and how to perform taste recordings. We also present some representative data obtained by recording from subsets of Drosophila sensilla, as well as some common issues and potential solutions that may be encountered when using this technique.

<table border="0" cellpadding="0" cellspacing="0" width="783">
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		<tr height="84">
			<td height="84" style="height:84px;width:168px;">Stereo Zoom Microscope</td>
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			<td style="width:219px;">capable of ~150x magnification with long working distance table mount stand</td>
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			<td height="42" style="height:42px;width:168px;">Anti-vibration Table</td>
			<td style="width:187px;">Kinetic Systems</td>
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			<td>Model #625-0930</td>
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			<td height="42" style="height:42px;width:168px;">Reference Electrode Holder</td>
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			<td style="width:211px;">ESP/W-F10N</td>
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			<td height="42" style="height:42px;width:168px;">Digital Acquisition System</td>
			<td style="width:187px;">Syntech</td>
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		<tr height="21">
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			<td style="width:187px;">Syntech</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:168px;">Tasteprobe Amplifier</td>
			<td style="width:187px;">Syntech</td>
			<td style="width:211px;">DTP-1</td>
			<td>Tasteprobe</td>
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			<td style="width:187px;">Grainger</td>
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			<td>Any brand is fine</td>
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			<td>Generic</td>
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			<td style="width:187px;">World Precision Instruments</td>
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			<td height="21" style="height:21px;width:168px;">Personal Computer</td>
			<td style="width:187px;">Dell&#160;</td>
			<td style="width:211px;">Vostro</td>
			<td>Check for compatibility with digital acquisition system and software</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:168px;">Acquisition Software</td>
			<td style="width:187px;">Syntech</td>
			<td style="width:211px;">Autospike</td>
			<td>Autospike works with IDAC-4; alternatively, use Labview with NI-6251</td>
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		<tr height="42">
			<td height="42" style="height:42px;width:168px;">Aluminum Foil and/or Faraday Cage</td>
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			<td style="width:187px;"></td>
			<td style="width:211px;"></td>
			<td>See recipe in protocol section</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:168px;">Tricholine citrate, 65%&#160;</td>
			<td style="width:187px;">Sigma</td>
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			<td>Assembled from P1000 pipette tips, flexible plastic tubing, and mesh</td>
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</table>

electrophysiological recording from drosophila labellar taste sensilla

The peripheral taste response of insects can be powerfully investigated with electrophysiological techniques. The method described here allows the researcher to measure gustatory responses directly and quantitatively, reflecting the sensory input that the insect nervous system receives from taste stimuli in its environment. This protocol outlines all key steps in performing this technique. The critical steps in assembling an electrophysiology rig, such as selection of necessary equipment and a suitable environment for recording, are delineated. We also describe how to prepare for recording by making appropriate reference and recording electrodes, and tastant solutions. We describe in detail the method used for preparing the insect by insertion of a glass reference electrode into the fly in order to immobilize the proboscis. We show traces of the electrical impulses fired by taste neurons in response to a sugar and a bitter compound. Aspects of the protocol are technically challenging and we include an extensive description of some common technical challenges that may be encountered, such as lack of signal or excessive noise in the system, and potential solutions. The technique has limitations, such as the inability to deliver temporally complex stimuli, observe background firing immediately prior to stimulus delivery, or use water-insoluble taste compounds conveniently. Despite these limitations, this technique (including minor variations referenced in the protocol) is a standard, broadly accepted procedure for recording Drosophila neuronal responses to taste compounds.

Figure 5A shows the response of an L sensillum to a sugar, sucrose. The same sensillum does not respond to a bitter compound, berberine. Figure 5B shows that an I type sensillum, which contains a bitter responsive neuron, displays larger amplitude spikes in response to berberine, and smaller amplitude spikes in response to sucrose. L sensilla display a minimal background response to the solvent control, TCC, while I sensilla display virtually no response to TCC (Figure 5...

Watch this Scientific Journal Video about Electrophysiological Recording From Drosophila Labellar Taste Sensilla at JoVE.com

Electrophysiological Recording From Drosophila Labellar Taste Sensilla

This protocol describes extracellular recording of the action potential responses fired by labellar taste neurons in Drosophila.

Electrophysiological Recording From Drosophila Labellar Taste Sensilla

The peripheral taste response of insects can be powerfully investigated with electrophysiological techniques. The method described here allows the ...

electrophysiological-recording-from-drosophila-labellar-taste-sensilla

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JoVE Journal

Neuroscience

16.1K Views.  Yale University. This protocol describes extracellular recording of the action potential responses fired by labellar taste neurons in Drosophila.