JoVE Logo
Faculty Resource Center

Sign In

A Method of Permeabilization of Drosophila Embryos for Assays of Small Molecule Activity

DOI :

10.3791/51634-v

July 13th, 2014

July 13th, 2014

12,176 Views

1Department of Environmental Medicine, University of Rochester School of Dentistry and Medicine

Introduction of small molecules to the developing Drosophila embryo offers great potential for characterizing biological activity of novel compounds, drugs, and toxins as well as for probing fundamental developmental pathways. Methods described herein outline steps that overcome natural barriers to this approach, expanding the utility of the Drosophila embryo model.

Tags

Drosophila Embryo

-- Views

Related Videos

article

Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos

article

Method for Culture of Early Chick Embryos ex vivo (New Culture)

article

Neurocircuit Assays for Seizures in Epilepsy Mutants of Drosophila

article

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

article

In-vivo Centrifugation of Drosophila Embryos

article

Upright Imaging of Drosophila Embryos

article

Large Scale Zebrafish-Based In vivo Small Molecule Screen

article

Isolation and Purification of Kinesin from Drosophila Embryos

article

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries

article

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved