JoVE Logo

Sign In

A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture

29.7K Views

09:18 min

August 11th, 2014

DOI :

10.3791/51765-v

August 11th, 2014


Explore More Videos

Keywords Organoid Culture

Chapters in this video

0:05

Title

1:17

Crypt Isolation

3:39

Virus Production

5:15

Retroviral Transduction

6:34

Spinoculation and Plating

8:01

Results: Representative Organoid Images

8:49

Conclusion

Related Videos

article

09:16

High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor

9.9K Views

article

07:37

Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning

8.1K Views

article

09:09

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

13.5K Views

article

10:12

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

8.0K Views

article

07:59

Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

2.9K Views

article

08:22

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

5.3K Views

article

08:01

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators

1.2K Views

article

10:58

A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

30.5K Views

article

09:31

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

17.5K Views

article

07:49

Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

10.6K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved