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30.9K Views
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10:27 min
May 15th, 2015
DOI :
10.3791/52793-v
Chapters
0:05
Title
2:02
Harvesting and Freezing Tissue
4:12
Embedding Tissues in OCT
6:12
Preparing Sections and Identifying Mid-belly Region Muscles
7:44
Notes on Staining and Mounting
8:53
Results
9:37
Conclusion
Summary
Here we demonstrate the most efficient methods for freezing, embedding, cryosectioning, and staining of muscle biopsies to avoid freezing artifacts.
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