August 9th, 2015
•A method for surface-spreading chromosomes from budding yeast is presented. This method is derived from a method previously described by Loidl and Klein. In addition, we demonstrate a procedure for immunostaining of spread chromosomes.
Tags
Related Videos
Purification of Mitochondria from Yeast Cells
Yeast Colony Embedding Method
High-throughput Yeast Plasmid Overexpression Screen
Competitive Genomic Screens of Barcoded Yeast Libraries
Chromatographic Purification of Highly Active Yeast Ribosomes
Immunostaining of Dissected Zebrafish Embryonic Heart
Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved