Compound of Interest (COI)-loaded Microsphere Preparation
3:01
COI-loaded Islet Treatment and Intact Islet Dispersion
5:40
Dissociated Islet Immunofluorescent Labeling
7:07
Results: Representative Quantification and Analysis of β-cell Proliferation
8:11
Conclusion
Transcript
The overall goal of this protocol is to use Poly(lactic-co-glycolic Acid)or PLGA, microspheres, to deliver compounds of interest to cultured intact pancreatic islets to study the effects of individual compounds on adult pancreatic beta cell prolif
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Here, we present methodology to generate and administer compound of interest-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres to intact mouse islets in culture with subsequent immunofluorescence analysis of β-cell proliferation. This method is suitable for determining the efficacy of candidate β-cell mitogens.