Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

6.8K Views

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08:10 min

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March 21st, 2019

DOI :

10.3791/56924-v

March 21st, 2019

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Ana C. Silva¹^,²^,³^,⁴, Maria J. Oliveira¹^,²^,⁵, Todd C McDevitt⁴^,⁶, Mário A. Barbosa¹^,²^,³, Diana S. Nascimento¹^,², Perpétua Pinto-do-Ó¹^,²^,³

¹i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, ²INEB - Instituto de Engenharia Biomédica, Universidade do Porto, ³Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, ⁴Gladstone Institute of Cardiovascular Disease, ⁵Faculty of Medicine, University of Porto, ⁶University of California San Francisco

Summary

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Keywords Decellularization

Sodium Dodecyl Sulfate

Chapters in this video

0:04

Title

0:44

Tissue Cryopreservation

2:05

Tissue Decellularization

3:53

Decellularization Efficacy Assessment

5:17

Cell Seeding onto Decellularized Scaffolds

6:31

Results: Analysis of Decellularized Scaffold Repopulation

7:34

Conclusion

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