Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning

8.1K Views

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07:37 min

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May 10th, 2019

DOI :

10.3791/57040-v

May 10th, 2019

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Lingling Xian¹, Lionel Chia¹^,⁵, Dan Georgess², Li Luo¹, Shuai Shuai¹, Andrew J. Ewald³^,⁴, Linda M.S. Resar¹^,⁴^,⁵^,⁶

¹Department of Medicine, Division of Hematology, Johns Hopkins University School of Medicine, ²Department of Natural Sciences, School of Arts & Sciences, Lebanese American University, ³Department of Cell Biology, Johns Hopkins University School of Medicine, ⁴Department of Oncology, Johns Hopkins University School of Medicine, ⁵Department of Pathology, Johns Hopkins University School of Medicine, ⁶Institute for Cell Engineering, Johns Hopkins University School of Medicine

Summary

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Keywords Genetic Engineering

Mouse Intestinal Organoids

Magnetic Nanoparticle Transduction

Intestinal Epithelium

Developmental Processes

Chapters in this video

0:04

Title

1:29

Genetic Engineering of Organoids by Viral Transduction

2:26

Seeding of Infected Organoid Fragments

3:44

Organoid Cryosection in Basement Membrane Matrix

5:32

Results: Efficient Viral Transduction of Intestinal Organoids

6:21

Conclusion

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