Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
12.3K Views
•
10:27 min
•
October 21st, 2018
DOI :
October 21st, 2018
•Chapters in this video
0:04
Title
0:54
Determine Neutron Contrast Match Points and Necessary Contrast for Protein Measurement
2:05
Express and Purify the Membrane Protein of Interest
3:25
Collect SANS Data and Reduce SANS Data from 2D Image to 1D Plot
4:48
Analyze Data for Structural Parameters of the Scattering Particle
7:06
Create Ab Initio Models from the SANS Data
8:17
Results: Visualization of the Experimental SANS Envelope and Overlay with High-resolution Model using PyMOL
9:40
Conclusion
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