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Choice and No-Choice Bioassays to Study the Pupation Preference and Emergence Success of Ectropis grisescens

DOI :

10.3791/58126-v

October 30th, 2018

October 30th, 2018

6,039 Views

1Guangdong Key Laboratory for Innovation Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, 2College of Agriculture, South China Agricultural University, 3Tea Research Institute, Chinese Academy of Agricultural Sciences, 4College of Horticulture, South China Agricultural University, 5College of Coast and Environment, Louisiana State University

Here, we present a protocol to investigate the pupation preference of mature larvae of Ectropis grisescens in response to soil factors (e.g., substrate type and moisture content) using choice bioassays. We also present a protocol of no-choice bioassays to determine the factors that affect the pupation behaviors and survivorship of E. grisescens.

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