Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
8.8K Views
•
07:41 min
•
October 17th, 2018
DOI :
October 17th, 2018
•Alex Y. Lin1,2, Yifu Ding1,2,3, Daniel J. Vanselow1,2, Spencer R. Katz1,2,3, Maksim A. Yakovlev1,2, Darin P. Clark4, David Mandrell5, Jean E. Copper1,2, Damian B. van Rossum1,2, Keith C. Cheng1,2
1The Jake Gittlen Laboratories for Cancer Research, Penn State College of Medicine, 2Division of Experimental Pathology, Department of Pathology, Penn State College of Medicine, 3Medical Scientist Training Program, Penn State College of Medicine, 4Center for In Vivo Microscopy, Duke University Medical Center, 5KTM Research
Chapters in this video
0:04
Title
1:05
Fish Fixation
1:48
Dehydration and Staining
2:24
Infiltration
3:07
Embedding
5:18
Results: Successful Embedding Using Custom-designed Adapter
7:01
Conclusion
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