Cell Plating and Multi-plate Reader Imaging Cytometer Setup
3:41
High-throughput Fluorescence-based Assay and Cell Enumeration
7:29
Results: Representative Plasma Membrane Efficiency Resealing in Mammalian Cells
9:15
Conclusion
Transcript
This assay was developed to address key questions in the field of plasma membrane biology and to establish how efficiently damaged cells can reseal their plasma membrane. This technique can be used to measure the efficiency of plasma membrane rese
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Here we describe a high-throughput fluorescence-based assay that measures the plasma membrane resealing efficiency through fluorometric and imaging analyses in living cells. This assay can be used for screening drugs or target genes that regulate plasma membrane resealing in mammalian cells.