LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

13.2K Views

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08:45 min

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November 17th, 2018

DOI :

10.3791/58564-v

November 17th, 2018

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Portia Ritter*¹^,², Keyvan Yousefi*¹^,³, Juliana Ramirez⁴, Derek M. Dykxhoorn⁴^,⁵, Armando J. Mendez⁶, Lina A. Shehadeh¹^,²^,⁷^,⁸

Summary

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Keywords LDL Cholesterol Uptake

Live Cell Imaging

Cell Health Monitoring

HepG2 Cells

HK2 Cells

HCAECs

PHrodo Red Labeled LDL

Lipoprotein Deficient Serum

Chapters in this video

0:04

Title

0:36

Cell Seeding in 24-well Plate

2:54

Live Cell Analysis

3:51

Analysis Parameter Setup

5:41

Analysis and Data Processing

7:06

Results: Measuring LDL Cholesterol Uptake in Various Cells Using Live Cell Imaging

8:05

Conclusion

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