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LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

DOI :

10.3791/58564-v

•

8:45 min

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November 17th, 2018

November 17th, 2018

•
12,637 Views
1Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine

This protocol provides an efficient approach to measuring LDL cholesterol uptake with real time influx rates using a live cell imaging system in various cell types. This technique provides a platform to screen the pharmacological activity of compounds affecting LDL influx while monitoring for cell morphology and hence potential cytotoxicity.

Tags

LDL Cholesterol Uptake Assay
Live Cell Imaging Analysis
Cell Health Monitoring
LDL Cholesterol Transport
Toxic Compounds Detection
Cell Morphology Monitoring
Media Aspiration
Cell Washing
Trypsin EDTA Solution
Incubation At 37 Degrees Celsius
Trypsin Neutralization
Fetal Bovine Serum FBS
Centrifugation
Cell Resuspension
Cell Counting
Optimized Cell Density
24 well Plate Incubation

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