T Cell-depleted Bone Marrow Reconstitution (Day 1)
3:58
T Cell Transfer (Day 2)
5:19
Graft Versus Host Disease (GvHD)-related Lesion Scoring
6:46
Results: Representative GvHD Scoring
8:56
Conclusion
Transcript
Mini-endoscopic evaluation of the colon in live mice provides key experimental support for investigating intestinal manifestations of acute graph-versus-host disease, GvHD, upon allogenic, hematopoietic stem cell transplantation. The non-invasive
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Here, we present a protocol that describes allogeneic hematopoietic stem cell transplantation and allows repetitive mini-endoscopic evaluations of the distal colon in situ for the presence, characteristics, and severity of colonic inflammation within live mice suffering from intestinal graft-versus-host disease.