A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
33.1K Views
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07:53 min
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January 9th, 2019
DOI :
January 9th, 2019
•Javier A. Jaimes*1,2, Jean K. Millet*1,3, Monty E. Goldstein1,4, Gary R. Whittaker1, Marco R. Straus1
1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 3Virologie et Immunologie Moléculaires, Domaine de Vilvert, INRA, 4Department of Cell Biology and Molecular Genetics, University of Maryland
Chapters in this video
0:04
Title
0:31
Designing and Preparing the Peptides
2:06
Preparing the Fluorescence Plate Reader
3:11
Preparing the Assay
4:30
Data Analysis
5:37
Results: Mutations in the Cleavage Site Alter Activity of Furin and Cleavage Efficiency
7:26
Conclusion
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