Results: Neuroarchitectural Analysis of Neurons Overexpressing a Neocortical Patterning Gene
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Conclusion
Transcript
The overall goal of this technique is the assessment of the effect of gene expression manipulations on cortical neuronal architecture in vivo. Our first key advantage of this procedure over those based on a neutral electroporation, is the fine con
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Based on in vitro lentiviral engineering of neuronal precursors, their co-transplantation into wild-type brains and paired morphometric evaluation of "test" and "control" derivatives, this method allows accurate modeling of in vivo gene control of neocortical neuron morphology in a simple and affordable way.