We wish to thank Paul Dressel for his invaluable support in graphical design and preparation of figures.

All procedures were approved by and followed guidelines of the University at Buffalo Institutional Animal Care and Use committee.
1. Fluid percussion injury
<ol>
	<li>Wear a lab coat or surgical gown, surgical mask, surgical gloves, and head covering and sterilize all tools and materials that contact the surgical site.</li>
	<li>Anesthetize a 10-12-week-old, male, Wistar rat (350-400 g) with 3% isoflurane and 1 L/min oxygen in an induction chamber of appropriate size for rats. Remove the rat from the induction chamber and move it to the prep area once it is unconscious. Put the sterile ophthalmic ointment into both eyes.</li>
	<li>Shave the hair&#160;on the rat&#8217;s head with electric clippers with a #40 blade from just above the eyes to the caudal base of the ears to produce enough surgical field. Remove any loose, clipped hair from the site.</li>
	<li>Clean the surgical site by applying 2% chlorohexidine scrub to the shaved scalp followed by 70% ethanol. Start at the center and move outward in concentric circles away from the incision site. Repeat this process 3 times. Apply Betadine solution to the site in the same fashion and allowed to dry.&#160;</li>
	<li>Place the anesthetized rat into the stereotaxic frame and maintain anesthesia at 2-3% isoflurane-1 L/min oxygen via nosecone. Check for loss of withdrawal reflex of hindlimb and loss of palpebral reflex to ensure the rat is in a surgical plane of anesthesia.</li>
	<li>Monitor the respiratory rate, heart rate, body temperature and oxygen saturation throughout the surgery. Maintain heart rate between 300-400 bpm, and SpO2 above 90%. 
	NOTE: A pulse-oximeter attached to a rear foot can be used to provide the constant read out of heart rate and SpO2. A heart rate above 400 bpm indicates the rat is not sufficiently anesthetized. A self-regulating warming pad, coupled to a rectal thermometer, set at 37 &#176;C, can be positioned under the rat throughout the surgery to maintain body temperature.A stereomicroscope with a light source in combination with an optic fiber lamp are helpful for visualizing the procedure.</li>
	<li>Use a 23 g needle to inject 0.5% bupivacaine hydrochloride intradermally into the scalp at the incision site for local analgesia 10 -&#160;15 minutes prior to making an incision.&#160;</li>
	<li>Make a 1.5-2.5 cm midline incision through the skin and muscle of the scalp using a #10 scalpel blade. Retract the skin and muscle to expose the skull and provide a clear surgical field. Reflect the underlying fascia and fatty tissue away from the bone with sterile cotton swabs. 
	NOTE: An electric cautery unit is useful for achieving&#160;quick hemostasis.</li>
	<li>Shave down the lateral ridge of the left parietal bone using a surgical curette to produce a smooth flat surface so that the base of the female-female Luer lock hub can rest flush with the skull.</li>
	<li>Irrigate the skull surface and surrounding tissues with 2.0 mg/mL gentamicin solution in sterile saline. Blot excess solution with a sterile swabs.</li>
	<li>Apply 3% hydrogen peroxide to the skull to dry the bone.&#160; 
	NOTE: If the bone is not sufficiently dry the dental cement will not adhere properly and form a solid seal.</li>
	<li>Create a 5 mm diameter craniectomy site through the left parietal bone.&#160; 
	NOTE: A trephine bit placed into a power drill attached to the stereotactic frame can be helpful to initiate the craniectomy. Use a hand drill with a 5 mm diameter trephine to slowly finish the craniectomy through the remaining bone. When close to completing the craniectomy, rotate the trephine in reverse to prevent rupture of the underlying dura mater. There will be a thinning of the skull around the perimeter of the disk and the skull flap will feel loose when pressed lightly.</li>
	<li>Remove the bone flap with the surgical curette and smooth tissue forceps. 
	NOTE: Some bleeding may occur, but hemostasis can be quickly achieved by applying gentle pressure with sterile cotton swabs.</li>
	<li>Use a stereomicroscope and illumination to visually inspect the dura for any signs of rupture.&#160; A thin rim of bone will remain around the circumference of the craniectomy site. &#160;Gently remove this rim with smooth tissue forceps taking care not to rupture the dura.&#160;</li>
	<li>Swab the skull with 70% ethanol to remove any bone dust and to dry the skull.</li>
	<li>Apply a thin layer of cyanoacrylate gel glue around the bottom edge of the Luer lock hub and secure it to the skull over the craniectomy without obstructing the opening. Use caution not to bring the glue in contact with the dura. Further, seal the Luer lock in place with an additional thin layer of glue around the outside base of the hub.</li>
	<li>Prepare a slurry of dental cement. Apply the cement to the surface of the skull around and over the base of the Luer lock hub to secure it in place.</li>
	<li>Fill the Luer lock hub with a sterile preservative free artificial cereberal spinal fluid (CSF) solution (pH 7.4) using a syringe and needle so that a convex bolus of saline can be seen above the top of the rim. 
	NOTE: The solution will keep the dura moist as the dental cement dries as well as serves as an indication of the integrity of the seal. If the solution level falls at all, that is an indication of a leak in the system and the Luer lock must be removed and replaced.</li>
	<li>Once the dental cement is completely cured, discontinue gas anesthesia and remove the rat from the stereotaxic frame.</li>
	<li>Place the rat on a platform next to the FPI device.</li>
	<li>The FPI device has a curved metal tip that extends from the pressure transducer at the end of the fluid reservoir. Secure a 12 cm length of pressure tubing to the end of the curved tip with the opposite end terminating in a 2 cm male Luer lock twist connector. Secure the rat to the FPI device by connecting the female end of the hub on the rat&#39;s skull to the male connector. 
	NOTE: Ensure the connection is tightly secured and that all air bubbles have been removed from the system.</li>
	<li>Place the animal in sternal recumbency and repeatedly check for return of withdrawal reflex. As soon as the rat regains withdrawal reflex but is still sedated, release the pendulum of the FPI device to cause a single 20 ms pressure pulse and induce injury. 
	NOTE: It is important to not induce the injury while the animal is deeply anesthetized as this tends to cause increased mortality due to neurogenic-induced pulmonary edema. All devices show variability. However, on the device used for this experiment, a 17&#176; angle placement of the hammer produces a 2.2 - 2.3 atmospheric pressure pulse. Uninjured, sham animals undergo all of the same procedures with the exception of the actual fluid pulse to the induce injury.</li>
	<li>Immediately disconnect the rat from the FPI device after injury, place it in sternal recumbency, and provide supplemental oxygen (1 L/min) via a nose cone until spontaneous breathing returns. Apnea is an anticipated consequence of the injury. If necessary, provide periodic manual breaths via a bag valve mask until the rat begins to spontaneously breathe on its own. 
	NOTE: Typically, apnea lasts less than 2 min. A transient rapid rise in heart rate (&#62;500 bpm) is observed immediately after the administration of the pressure pulse due to a catecholamine burst. This can be monitored with a pulse oximeter attached to the rat&#8217;s foot and can serve as a possible indicator that a severe injury has occurred.</li>
	<li>Monitor the rat continuously and record the time of return of righting reflex (stable ambulation on all four limbs).</li>
	<li>The magnitude of the atmospheric pressure pulse for each rat should be within &#177; 0.05 atmospheres of each other. Confirm that each of the pressure pulse produces a smooth signal on the oscilloscope with consistent amplitude and duration. 
	NOTE: A noisy signal may indicate air bubbles in the system that must be removed prior to delivering the injury pulse. Atmospheric pressure pulses that produce a severe injury, in this experiment, are those that typically result in animal righting times of 30-60 min. This range of righting times are associated with a mortality rate of approximately 40-50%).</li>
	<li>Administer 10 mL of prewarmed saline subcutaneously as a supportive care.</li>
	<li>Return the rat to its home cage and allow it to recover for at least 4 h. 
	NOTE: Increased mortality has been observed when the rats are placed immediately back under anesthesia.</li>
</ol>
2. Implantation of cortical EEG electrodes and video-EEG recording
<ol>
	<li>At 4 h after injury, anesthetize the rat as previously described and place it back into the stereotactic frame to remove the Luer lock hub and dental cement.&#160; 
	NOTE: The hub and cement will easily snap off with moderate pressure. When removing the hub, check carefully for any rupture or damage to the dura. Immediatly&#160;euthanize any animal with damage to the dura.</li>
	<li>Apply a small drop of 0.5% bupivacaine hydrochloride to the skull in each of the locations where 5 pilot holes are to be drilled (see Figure 1).</li>
	<li>Drill pilot holes through the skull with a hand-held 0.1 mm drill bit.</li>
	<li>Secure a stainless-steel electrode screw into each pilot hole at the following locations: a reference screw is placed caudal to the lambda over the cerebellum. Recording electrodes are placed: 1) over the hemisphere ipsilateral and rostral to the craniectomy; 2) over the hemisphere ipsilateral and caudal to the craniectomy; 3) over the hemisphere contralateral and rostral to the craniectomy; 4) over the hemisphere contralateral and caudal to the craniectomy.</li>
	<li>Swab the skull with 70% ethanol to remove any bone dust.</li>
	<li>Cover the craniectomy site with a thin layer of sterile bone wax to cover the exposed dura.&#160;</li>
	<li>Connect an electrode array to the 5 EEG electrodes by wrapping the exposed end of a color-coded electrode wire tightly around its designated stainless-steel electrode screw. &#160; 
	NOTE: The opposite ends of each electrode wire is positioned into a specific, designated location within the pedestal connector.</li>
	<li>Prepare a slurry of bone cement.</li>
	<li>Collect the electrode wires into a coil underneath the pedestal and secure the wires and pedestal into place with bone cement. Hold the pedestal in position until the bone cement has cured. 
	NOTE: The bone must be particularly dry and void of any residual blood in order to achieve proper adhesion and prevent premature removal of the transmitter.&#160;</li>
	<li>Attach the wireless transmitter with fresh batteries to the pedestal before removing the animal from the stereotactic frame.</li>
	<li>Place the animal in its home cage and place the cage in proximity to the receiver and in view of a designated video camera. Initiate video/EEG recording.</li>
</ol>
3. Collection of video-EEG recordings
<ol>
	<li>Prior to collecting EEG signals, do a frequency sweep of the room where rats will be housed for EEG collection to identify any potential interfering frequencies to prevent the collection of EEG recording with any frequency that has background noise.</li>
	<li>Set all transmitters to specific frequencies that are free of interference.</li>
	<li>Set the sampling frequency and the input range of each programmable transmitter. 
	NOTE: This can be done using a smart tool provided by the system manufacturer. Transmitters can sample at a maximum rate of 1000 Hz, and a maximum input range of &#177;10 mV. In this experiment, EEG recordings between 0.5 Hz to 30 Hz were&#160;analyzed. Therefore, the sample rate was set at 250 Hz. We typically observe&#160;Amplitudes of less than 1 mV. Therefore, the set input range was at &#177;2 mV.</li>
	<li>Use EEG collection software provided by the manufacturer to continuously record video-EEG beginning on the day of injury linking each wireless transmitter via a unique frequency to a specific receiver. 
	NOTE: Each transmitter receiver pair is capable of monitoring 4 monopolar EEG channels, and acceleration in the X, Y and Z planes. EEG data can be written to a storage server. The video data should be saved on a NAS device linked to the storage server. The EEG analysis software synchronizes the video and EEG recording based on the time maintained by the storage server.</li>
	<li>Use the video collection software to record video of each rat with its own 2 MP resolution camera (1920 x 1080) configured to record at 30 frames/s. 
	NOTE: Each camera has its own infrared illumination for video collection at night.</li>
	<li>Configure the system to automatically save all video and EEG recordings to a storage server every 24 h. The videos produce rather large files.</li>
</ol>
4. Video/EEG analysis
<ol>
	<li>Synchronize the video with each EEG recording at 1/10 s resolution. Do this by using the system manufacturers video/EEG analysis software that creates a metafile with the stamp of the precise time of both of the EEG and the video.</li>
	<li>Manually screen through EEG recordings to identify index events that define seizure activity.</li>
	<li>Using the video/EEG analysis software and index EEG events, create a configuration file that uses key parameters (i.e., power in specific frequency bands, the ratio of frequency bands to the total power, acceleration threshold, etc.) to define the characteristics of the potential seizure events.</li>
	<li>Run the EEG analysis software to identify potential regions of EEG recording that qualify based on the parameters selected in the configuration file. 
	NOTE: The EEG analysis software allows for automatic seizure detection and highlights regions of interest in the EEG signals and provides FFT power spectrum analysis across the signal.</li>
	<li>Confirm potential convulsive seizures by using video recordings collected during acquisition, which are synchronized with each rat&#8217;s respective EEG recordings.</li>
</ol>

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Chelasea R Richardson is an employee of emka Scientific, the supplier of this wireless telemetry system described.

Considerable variability has been reported between laboratories regarding the specific parameters and methods used for the FPI TBI model 14,26,27,28. These inconsistencies have resulted in conflicting results and make it difficult to harmonize efforts and outcomes between labs. Here, we have presented a detailed methodology describing our approach to long-term, continuous recording of video/EEG to monitor for post-traumatic epileptiform activity. A number of steps are critical to generating reproducible results with the described method.
First, given that the incidence of post-traumatic epilepsy correlates with injury severity, apply conditions that result in the most severe TBI. Specifically, use a 5 mm craniectomy to ensure that a sufficiently large area of dura is exposed. In addition, secure a female-female Luer lock device onto the surface of the skull, with the opening placed directly over the craniectomy. This differs from other labs that have used a smaller craniectomy (3 mm) and/or placed a modified needle hub inside the craniectomy, which effectively reduces the opening size. By placing the Luer lock outside of the craniectomy, the 5mm opening is maintained. These specific parameters impact the overall force applied to the dura. The atmospheric pressure applied to the dura also has a major impact on the severity of injury observed. Unfortunately, atmospheric pressure is highly variable and appears to be device dependent. Some labs have reported applying a pressure pulse of 8 - 10 ms18. In contrast, the method described here results in a 20 ms pressure pulse. This is consistent with other labs that appear to generate more severe injury 14,28. It is clear that the injury-inducing pressure pulse is a parameter that shows considerable variability between labs and must be empirically defined. However, injury severity may be determined based on a combination of mortality rates (40-50%), righting reflex times (&#62;30 min)26. It is also critical that only animals with an intact dura be included in the study. In addition, if the craniectomy is occluded by any glue or cement such that part of the dura beneath the craniectomy is not exposed to the full force of the fluid pressure pulse, then the animal should be eliminated from the study.&#160; Also, excess glue beneath the Luer lock can adhere to the dura and remove it with the cement cap even after a successful injury.&#160; Finally, the smooth shape of the pressure pulse curve on the oscilloscope trace gives the indication that there are no air bubbles in the fluid chamber and indicates the plunger is moving without impedance.
Anesthesia is another critical factor that must be controlled. Isoflurane exposure should be kept to the lowest levels possible to maintain a surgical plane of anesthesia. Rats exposed to higher levels of isoflurane or for long durations are more likely to develop neurogenic-induced pulmonary edema. Preparation of the skull represents another critical aspect of the method. Particularly, drying the skull and removing any bone dust helps to prevent the rats from removing the transmitter prematurely.
The placement of screws and the connection of the EEG wires are obviously critical to producing consistently reproducible recordings. It is important that the screws are not placed too deeply as to induce a lesion on the brain. The bone flap recovered from the craniectomy of adult (12 weeks old) male Wistar rats is consistently 2 mm thick. Use EEG electrode screws with a 2.5 mm shaft. It is helpful to use the tips of curved mosquito hemostatic forceps as a spacer to ensure that the screws only extend to the base of the bone and do not protrude into the brain.
The approach presented here does have some limitations. Batteries must be changed on a regular basis. The frequency of battery changes depends on the sampling rate. Batteries are typically changed once a week for a sampling rate of 1000 Hz. This time frame can be extended by reducing the sampling rate. The system is also limited to recording from four monopolar EEG electrodes. However, this provides two channels per hemisphere and can differentiate between focal and generalized events and can differentiate between anterior and posterior changes. Despite these limitations, this approach provides a reasonable method to conduct continuous video/EEG monitoring and detection of epileptiform changes following severe TBI.
The method described here results in both electrographic and convulsive seizures within one month following TBI. Therefore, this approach provides a reasonable time frame in which to study potential therapeutics for preventing epileptogenesis following severe TBI. This approach also provides a method to investigate the molecular mechanisms associated with PTE and may lead to the identification of potential biomarkers that can be used to identify patients who are most at risk of developing PTE.&#160;

In a 2015 report to Congress, the Centers for Disease Control reported that approximately 2.5 million people per year suffer traumatic brain injury (TBI) in the US1. It is estimated that TBI causes 20% of symptomatic epilepsies and 5% of all epilepsies2,3,4. In addition, about 20% of TBI patients develop post-traumatic epilepsy5. Importantly, chronic, recurrent seizures that occur as a consequence of TBI are often pharmacoresistant, increasing the burden of the disease6. The exact mechanisms that lead to post-traumatic epilepsy (PTE) remain unclear. However, several key epidemiology studies have examined the incidence and potential risk of developing post-traumatic epilepsy (PTE)2,4,7,8,9,10,11. These epidemiology studies each reinforced the correlation of injury severity with the risk of epileptogenesis.
Current methods that have been extensively used to identify novel anti-epilepsy therapies have relied heavily on models that use chemo-convulsants or electrical kindling to induce epilepsy12. Given the high incidence of pharmaco-resistance to drugs developed in these models by TBI patients, we hypothesize that TBI-induced seizures may be different from chemoconvulsant or kindling-induced seizures and may involve different pathways or processes of epileptogenesis. Therefore, a TBI model may be better suited for the development of treatments that are more effective to prevent post-traumatic epileptogenesis.
The fluid percussion injury (FPI) model of TBI has been used for decades and is a well-established method to investigate both TBI and PTE13,14,15,16,17,18. However, as we recently reviewed, there is a high degree of variability in the FPI methods reported across laboratories19,20. This lack of consistency between laboratories prevents reproducibility of preclinical findings and makes the interpretation of results a challenge. As a consequence, increased interest and efforts have been applied towards establishing a greater harmonization for these types of studies21,22,23,24.
In an effort to further increase the consistency and harmonization between laboratories focused on studying post-traumatic epileptogenesis, we provide here a detailed methodology of our approach. We have previously reported a 60% incidence of convulsive seizures within six weeks after severe TBI20. We now use this approach to monitor rats beginning the day of injury and continuously follow them 24 hours a day for up to 4 weeks. We have chosen to use a wireless telemetry system which affords several advantages. First, rats are able to freely move about their cage, and thus reduces stress. Second a reduction in signal noise as the rat serves as the ground. In addition, our current system employs an accelerometer which detects rapid movement in all three planes (X, Y and Z) and can be helpful to identify convulsive seizure events. Finally, the wireless telemetry system allows for easier management of rats such as supplemental saline injections, weighing and conducting neurological severity scores, which is complicated when rats are attached to a tether. However, this approach also has several limitations. First, the initial cost of a system to record from up to eight rats simultaneously can be in the range of $60,000. Second, power is limited by a battery source. This requires daily monitoring and replacement of batteries. The time required between battery changes can be influenced by the sampling rate. However, for a 1000 Hz sampling rate, batteries are typically changed once a week. The limited power supply also restricts the system to recording from only four EEG signals. Finally, signal drop out is limited but does occasionally occur. However, this approach provides a consistent and reliable method to monitor post-traumatic epileptogenesis and can aid in the identification of novel therapeutic treatments.

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use of a wireless video-eeg system to monitor epileptiform discharges following lateral fluid-percussion induced traumatic brain injury

The lateral fluid percussion injury (FPI) model is well established and has been used to study TBI and post-traumatic epilepsy (PTE). However, considerable variability has been reported for the specific parameters used in different studies that have employed this model, making it difficult to harmonize and interpret the results between laboratories. For example, variability has been reported regarding the size and location of the craniectomy, how the Luer lock hub is placed relative to the craniectomy, the atmospheric pressure applied to the dura and the duration of the pressure pulse. Each of these parameters can impact injury severity, which directly correlates with the incidence of PTE. This has been manifested as a wide range of mortality rates, righting reflex times and incidence of convulsive seizures reported. Here we provide a detailed protocol for the method we have used to help facilitate harmonization between studies. We used FPI in combination with a wireless EEG telemetry system to continuously monitor for electrographic changes and detect seizure activity.&#160; FPI is induced by creating a 5 mm craniectomy over the left hemisphere, between the Bregma and Lambda and adjacent to the lateral ridge. A Luer lock hub is secured onto the skull over the craniectomy. This hub is connected to the FPI device, and a 20-millisecond pressure pulse is delivered directly to the intact dura through pressure tubing connected to the hub via a twist lock connector. Following recovery, rats are re-anesthetized to remove the hub. Five 0.5 mm, stainless steel EEG electrode screws are placed in contact with the dura through the skull and serve as four recording electrodes and one reference electrode. The electrode wires are collected into a pedestal connector which is secured into place with bone cement. Continuous video/EEG recordings are collected for up to 4 weeks post TBI.

With this model, we induced severe TBI into adult, male, Wistar rats. Under the conditions we describe here, we typically observe mortality rates of 40-50%, and righting reflex times of 30 - 60 min as previously described20. We were able to collect video/EEG recordings 24 h/day beginning on the day of injury. A diagram showing the location of four monopolar EEG electrodes and a single reference electrode is shown in Figure 1A. Images which demonstrate the location and appearance of the TBI lesions expected with the conditions described here are shown in Figure 1B-D. Under the conditions described here, we consistently observe delta slowing within the first three days post TBI. Less severely injured rats exhibit unilateral, intermittent delta slowing (Figures 2C-D). In contrast, continuous, bilateral delta slowing is observed after more severe injuries (Figure 3C-D). Some degree of delta slowing was consistently observed in all TBI rats but was not detected in any sham operated (craniectomy only) control rats (Figures 2A-B; 3A-B). Extensive delta slowing was consistently observed during the first three days after injury in most TBI rats. Interestingly, rats typically show pronounced weight loss during the first three days post injury. Non-convulsive seizures are occasionally observed within the first week following TBI (Figure 4 C-D). Clinical seizures, presenting as spike clusters associated with rearing and falling as well as forearm clonus can be observed after 1-week post TBI (Figure 5C-D). Finally, Figure 6 presents representative images of occasional intermittent signal drop out and loss of signal due to battery failure.&#160;
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/59637/59637fig1.jpg" /> 
Figure 1. Location of craniectomy, electrode placement, and lesion. (A) shows a schematic diagram of the rat skull with the locations of the craniectomy (grey circle in the left hemisphere), four monopolar electrodes (Black dots; 1,2,3,4) located between the Bregma and Lambda and a reference electrode (Black dot, R) placed midline, posterior to the lambda; (B) shows coronal post-mortem T2 MRI scans with the location of the lesion identified by a red circle; (C) shows a 2-D map of the cortex where the location and size of the lesion is identified (blue region). (D) shows a Nissl stained coronal section with the lesion boxed, lesion is 100x magnified in image to the right. <a href="https://www.jove.com/files/ftp_upload/59637/59637fig1large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/59637/59637fig2.jpg" /> 
Figure 2. Unilateral, intermittent delta slowing collected on the day of a moderate TBI. (A) shows a 90 s EEG trace from a sham operated, uninjured control rat on the day of surgery. All four channels are presented. A 10 s long trace (taken from the boxed region) was extracted from the 3rd channel to better visualize the baseline EEG pattern. A 2048 ms EPOC section of this was then selected to be analyzed in the corresponding FFT. (B) FFT analysis of 2048 ms selected EPOC from the uninjured sham operated animal on the day of surgery. (C) shows a 90 s EEG trace, which demonstrates the intermittent, unilateral delta slowing pattern of a moderately injured animal on the day of injury. A 10 s long trace (taken from the boxed region) was extracted from the 3rd channel to better visualize the delta slowing EEG pattern. A 2048 ms EPOC section of this was then selected to be analyzed in the corresponding FFT. (D) FFT analysis of 2048 ms selected EPOC from the moderate TBI animal on the day of injury. 90 s EEG tracings, from top to bottom are biopotentials 1, 2, 3, 4, corresponding to their locations around the craniectomy site as seen in Figure 1. Grey vertical marks define 1 s intervals on the EEG traces. All EEG traces are shown on a scale of (&#177;500 &#181;V).&#160; Within FFT Analysis graphs, overall analyzed frequency range was 0.5-30 Hz. This was further broken down into 4 separate frequency bands of Delta (Yellow, 0.5-4 Hz), Theta (Purple, 4-8 Hz), Alpha (Red, 8-12 Hz), and Beta (Green, 12-30 Hz).&#160; %&#160; (Power) graph shown within the FFT analysis tells what percentage of the total power in the analyzed EPOC comes from each previously specified frequency band, allowing for further mathematical characterization of the EEG waveform patterns. <a href="https://www.jove.com/files/ftp_upload/59637/59637fig2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/59637/59637fig3.jpg" /> 
Figure 3. Bilateral, continuous delta slowing collected on the day of a severe TBI. (A) shows a 90 s EEG trace from a sham operated, uninjured control rat on the day of surgery. All four channels are presented.&#160; A 10 s long trace (taken from the boxed region) was extracted from the 3rd channel to better visualize the baseline EEG pattern. A 2048 ms EPOC section of this was then selected to be analyzed in the corresponding FFT. (B) FFT analysis of 2048 ms selected EPOC from the uninjured sham operated animal on the day of surgery. (C) shows a 90 s EEG trace, which demonstrates the continuous, bilateral delta slowing pattern of a severely injured animal on the day of injury.&#160; A 10 s long trace (taken from the boxed region) was extracted from the 3rd channel to better visualize the delta slowing EEG pattern. A 2048 ms EPOC section of this was then selected to be analyzed in the corresponding FFT. (D) FFT analysis of 2048 ms selected EPOC from the severe TBI animal on the day of injury. 90 s EEG tracings, from top to bottom are biopotentials 1, 2, 3, 4, corresponding to their locations around the craniectomy site as seen in Figure 1. Grey vertical marks define 1 s intervals on the EEG traces. All EEG traces are shown on a scale of (&#177; 500 &#181;V).&#160; Within FFT Analysis graphs, overall analyzed frequency range was 0.5-30 Hz.&#160; This was further broken down into 4 separate frequency bands of Delta (Yellow, 0.5-4 Hz), Theta (Purple, 4-8 Hz), Alpha (Red, 8-12 Hz), and Beta (Green, 12-30 Hz). % (Power) graph shown within the FFT analysis tells what percentage of the total power in the analyzed EPOC comes from each previously specified frequency band, allowing for further mathematical characterization of the EEG waveform patterns. <a href="https://www.jove.com/files/ftp_upload/59637/59637fig3large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/59637/59637fig4.jpg" /> 
Figure 4. Nonconvulsive electrographic seizure collected 3 days post severe TBI. (A) shows a 90 s EEG trace from a sham operated, uninjured control rat 3 days25 after surgery. All four channels are presented. A 10 s long trace (taken from the boxed region) was extracted from the 3rd channel to better visualize the baseline EEG pattern. A 2048 ms EPOC section of this was then selected to be analyzed in the corresponding FFT. (B) FFT analysis of 2048 ms selected EPOC from the uninjured sham operated animal on the day three25 after of surgery. (C) shows a 90 s EEG trace three 25 days post severe injury.&#160; This show building, fast spiking pattern present bilaterally and across all 4 collecting channels.&#160; A 10 s long trace (taken from the boxed region) was extracted from the 3rd channel to better visualize the spiking EEG pattern. A 2048 ms EPOC section of this was then selected to be analyzed in the corresponding FFT. (D) FFT analysis of 2048 ms selected EPOC from the severe TBI animal on the day of injury. &#160;90 s EEG tracings, from top to bottom are biopotentials 1, 2, 3, 4, corresponding to their locations around the craniectomy site as seen in Figure 1. Grey vertical marks define 1 s intervals on the EEG traces. All EEG traces are shown on a scale of (&#177; 500 &#181;V).&#160; Within FFT Analysis graphs, overall analyzed frequency range was 0.5-30 Hz.&#160; This was further broken down into 4 separate frequency bands of Delta (Yellow, 0.5-4 Hz), Theta (Purple, 4-8 Hz), Alpha (Red, 8-12 Hz), and Beta (Green, 12-30 Hz).&#160; % (Power) graph shown within the FFT analysis tells what percentage of the total power in the analyzed EPOC comes from each previously specified frequency band, allowing for further mathematical characterization of the EEG waveform patterns. <a href="https://www.jove.com/files/ftp_upload/59637/59637fig4large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 5" class="xfigimg" src="/files/ftp_upload/59637/59637fig5.jpg" /> 
Figure 5. Convulsive electrographic seizure collected 9 days post TBI. (A) shows a 90 s EEG trace from a sham operated, uninjured control rat nine (9) days after surgery. All four channels are presented. A 10 s long trace (taken from the boxed region) was extracted from the 3rd channel to better visualize the baseline EEG pattern. A 2048 ms EPOC section of this was then selected to be analyzed in the corresponding FFT. (B) FFT analysis of 2048 ms selected EPOC from the uninjured sham operated animal on the day nine (9) after of surgery. (C) shows a 90 s EEG trace nine (9) days post severe injury. This show building, fast spiking pattern present bilaterally and across all 4 collecting channels. A 10 s long trace (taken from the boxed region) was extracted from the 3rd channel to better visualize the spiking EEG pattern.&#160; A 2048 ms EPOC section of this was then selected to be analyzed in the corresponding FFT. (D) FFT analysis of 2048 ms selected EPOC from the severe TBI animal nine (9) days post injury. 90 s EEG tracings, from top to bottom are biopotentials 1, 2, 3, 4, corresponding to their locations around the craniectomy site as seen in Figure 1. Grey vertical marks define 1 s intervals on the EEG traces. All EEG traces are shown on a scale of (&#177; 500 &#181;V). Within FFT Analysis graphs, overall analyzed frequency range was 0.5-30 Hz. This was further broken down into 4 separate frequency bands of Delta (Yellow, 0.5-4 Hz), Theta (Purple, 4-8 Hz), Alpha (Red, 8-12 Hz), and Beta (Green, 12-30 Hz).&#160; %(Power) graph shown within the FFT analysis tells what percentage of the total power in the analyzed EPOC comes from each previously specified frequency band, allowing for further mathematical characterization of the EEG waveform patterns. <a href="https://www.jove.com/files/ftp_upload/59637/59637fig5large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 6" class="xfigimg" src="/files/ftp_upload/59637/59637fig6.jpg" /> 
Figure 6. Signal drop out. These are 3 separate examples of what signal drop out due to transmitter or receiver issues appears as on the EEG recording. (A) This is an example of intermittent dropout of the EEG signal on a recording.&#160; (B) This is an example of drop out due to battery failure during continuous wireless telemetry appears as on an EEG tracing. &#160;(C) Within the circled region, it can be seen that when the Quality of Signal (QoS) drops from 100 to 0, the EEG tracing becomes flattened and stagnant at 0 &#181;V.&#160; Grey vertical marks define 1 s intervals on the EEG traces. All EEG traces are shown on a scale of (&#177; 500 &#181;V). <a href="https://www.jove.com/files/ftp_upload/59637/59637fig6large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

Watch this Scientific Journal Video about Use of a Wireless Video-EEG System to Monitor Epileptiform Discharges Following Lateral Fluid-Percussion Induced Traumatic Brain Injury at JoVE.com

Use of a Wireless Video-EEG System to Monitor Epileptiform Discharges Following Lateral Fluid-Percussion Induced Traumatic Brain Injury

Here we present a protocol to induce severe TBI with the lateral fluid percussion injury (FPI) model in adult, male Wistar rats. We also demonstrate the use of a wireless telemetry system to collect continuous video-EEG recordings and monitor for epileptiform discharges consistent with post-traumatic epileptogenesis.&#160;

The lateral fluid percussion injury (FPI) model is well established and has been used to study TBI and post-traumatic epilepsy (PTE). However, ...

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25.4K Views.  Jacobs School of Medicine and Biomedical Science. Here we present a protocol to induce severe TBI with the lateral fluid percussion injury (FPI) model in adult, male Wistar rats. We also demonstrate the use of a wireless telemetry system to collect continuous video-EEG recordings and monitor for epileptiform discharges consistent with post-traumatic epileptogenesis. 

Video: Use of a Wireless Video-EEG System to Monitor Epileptiform Discharges Following Lateral Fluid-Percussion Induced Traumatic Brain Injury

Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

We present an operant system for the detection of pain in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) assesses pain behaviors in a more clinically relevant way by not relying on reflex-based measures of nociception. Food fasted, hairless (or shaved) rodents are placed into a Plexiglas chamber which has two Peltier-based thermodes that can be programmed to any temperature between 7 &deg;C and 60 &deg;C. The rodent is trained to make contact with these in order to access a reward bottle. During a session, a number of behavioral pain outcomes are automatically recorded and saved. These measures include the number of reward bottle activations (licks) and facial contact stimuli (face contacts), but custom measures like the lick/face ratio (total number of licks per session/total number of contacts) can also be created. The stimulus temperature can be set to a single temperature or multiple temperatures within a session. The OPAD is a high-throughput, easy to use operant assay which will lead to better translation of pain research in the future as it includes cortical input instead of relying on spinal reflex-based nociceptive assays.

Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior

We present a user-friendly, high-throughput operant system for the evaluation of pain behaviors in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) can assess pain through a reward/conflict paradigm thus providing a more humane way of testing. This protocol will yield more clinically relevant and translational data from rodents.

We present an operant  system for the detection of pain in awake, conscious rodents. The Orofacial  Pain Assessment Device (OPAD) assesses pain behaviors ...

Use of an Eight-arm Radial Water Maze to Assess Working and Reference Memory Following Neonatal Brain Injury

Working and reference memory are commonly assessed using the land based radial arm maze.&#160;However, this paradigm requires pretraining, food deprivation, and may introduce scent cue confounds. The eight-arm radial water maze is designed to evaluate reference and working memory performance simultaneously by requiring subjects to use extra-maze cues to locate escape platforms and remedies the limitations observed in land based radial arm maze designs. Specifically, subjects are required to avoid the arms previously used for escape during each testing day (working memory) as well as avoid the fixed arms, which never contain escape platforms (reference memory). Re-entries into arms that have already been used for escape during a testing session (and thus the escape platform has been removed) and re-entries into reference memory arms are indicative of working memory deficits. Alternatively, first entries into reference memory arms are indicative of reference memory deficits. We used this maze to compare performance of rats with neonatal brain injury and sham controls following induction of hypoxia-ischemia and show significant deficits in both working and reference memory after eleven days of testing. This protocol could be easily modified to examine many other models of learning impairment.

The eight-arm radial water maze is designed to evaluate reference and working memory performance simultaneously by requiring subjects to use extra-maze cues to locate escape platforms and remedies the limitations observed in land based radial arm maze designs.

Working and reference memory are commonly assessed using the land based radial arm maze.&#160;However, this paradigm requires pretraining, food ...

Compensatory Limb Use and Behavioral Assessment of Motor Skill Learning Following Sensorimotor Cortex Injury in a Mouse Model of Ischemic Stroke

Mouse models have become increasingly popular in the field of behavioral neuroscience, and specifically in studies of experimental stroke. As models advance, it is important to develop sensitive behavioral measures specific to the mouse. The present protocol describes a skilled motor task for use in mouse models of stroke. The Pasta Matrix Reaching Task functions as a versatile and sensitive behavioral assay that permits experimenters to collect accurate outcome data and manipulate limb use to mimic human clinical phenomena including compensatory strategies (i.e., learned non-use) and focused rehabilitative training. When combined with neuroanatomical tools, this task also permits researchers to explore the mechanisms that support behavioral recovery of function (or lack thereof) following stroke. The task is both simple and affordable to set up and conduct, offering a variety of training and testing options for numerous research questions concerning functional outcome following injury. Though the task has been applied to mouse models of stroke, it may also be beneficial in studies of functional outcome in other upper extremity injury models.

Mouse models have become increasingly popular in studies of behavioral neuroscience. As models advance, it is important to develop sensitive behavioral measures specific to the mouse. This protocol describes the Pasta Matrix Reaching Task, which is a skilled motor task for use in mouse models of stroke.

Mouse models have become increasingly popular in the field of behavioral neuroscience, and specifically in studies of experimental stroke. As models ...

Assessment of Murine Exercise Endurance Without the Use of a Shock Grid: An Alternative to Forced Exercise

Using laboratory mouse models, the molecular pathways responsible for the metabolic benefits of endurance exercise are beginning to be defined. The most common method for assessing exercise endurance in mice utilizes forced running on a motorized treadmill equipped with a shock grid. Animals who quit running are pushed by the moving treadmill belt onto a grid that delivers an electric foot shock; to escape the negative stimulus, the mice return to running on the belt. However, avoidance behavior and psychological stress due to use of a shock apparatus can interfere with quantitation of running endurance, as well as confound measurements of postexercise serum hormone and cytokine levels. Here, we demonstrate and validate a refined method to measure running endurance in na&#239;ve C57BL/6 laboratory mice on a motorized treadmill without utilizing a shock grid. When mice are preacclimated to the treadmill, they run voluntarily with gait speeds specific to each mouse. Use of the shock grid is replaced by gentle encouragement by a human operator using a tongue depressor, coupled with sensitivity to the voluntary willingness to run on the part of the mouse. Clear endpoints for quantifying running time-to-exhaustion for each mouse are defined and reflected in behavioral signs of exhaustion such as splayed posture and labored breathing. This method is a humane refinement which also decreases the confounding effects of stress on experimental parameters.

A method to assess exercise endurance in laboratory mice without the use of a shock grid is demonstrated. This method is a humane refinement that can decrease the confounding effects of stress on experimental parameters.

Using laboratory mouse models, the molecular pathways responsible for the metabolic benefits of endurance exercise are beginning to be defined. The most ...

Exploring the Use of Isolated Expressions and Film Clips to Evaluate Emotion Recognition by People with Traumatic Brain Injury

The current study presented 60 people with traumatic brain injury (TBI) and 60 controls with isolated facial emotion expressions, isolated vocal emotion expressions, and multimodal (i.e., film clips) stimuli that included contextual cues. All stimuli were presented via computer. Participants were required to indicate how the person in each stimulus was feeling using a forced-choice format. Additionally, for the film clips, participants had to indicate how they felt in response to the stimulus, and the level of intensity with which they experienced that emotion.

This paper describes how to implement a battery of behavioral tasks to examine emotion recognition of isolated facial and vocal emotion expressions, and a novel task using commercial television and film clips to assess multimodal emotion recognition that includes contextual cues.

The current study presented 60 people with traumatic brain injury (TBI) and 60 controls with isolated facial emotion expressions, isolated vocal emotion ...

Manipulation of Epileptiform Electrocorticograms (ECoGs) and Sleep in Rats and Mice by Acupuncture

Ancient Chinese literature has documented that acupuncture possesses efficient therapeutic effects on epilepsy and insomnia. There is, however, little research to reveal the possible mechanisms behind these effects. To investigate the effect of acupuncture on epilepsy and sleep, several issues need to be addressed. The first is to identify the acupoints, which correspond between humans, rats, and mice. Furthermore, the depth of insertion of the acupuncture needle, the degree of needle twist in manual needle acupuncture, and the stimulation parameters for electroacupuncture (EA) need to be determined. To evaluate the effects of acupuncture on epilepsy and sleep, a feasible model of epilepsy in rodents is required. We administer pilocarpine into the left central nucleus of the amygdala (CeA) to simulate focal temporal lobe epilepsy (TLE) in rats. Intraperitoneal (IP) injection of pilocarpine induces generalized epilepsy and status epilepticus (SE) in rats. Five IP injections of pentylenetetrazol (PTZ) with a one-day interval between each injection successfully induces spontaneous generalized epilepsy in mice. Recordings of electrocorticograms (ECoGs), electromyograms (EMGs), brain temperature, and locomotor activity are used for sleep analysis in rats, while ECoGs, EMGs, and locomotor activity are employed for sleep analysis in mice. ECoG electrodes are implanted into the frontal, parietal, and contralateral occipital cortices, and a thermistor is implanted above the cerebral cortex by stereotactic surgery. EMG electrodes are implanted into the neck muscles, and an infrared detector determines locomotor activity. The criteria for categorizing vigilance stages, including wakefulness, rapid eye movement (REM) sleep, and non-REM (NREM) sleep are based on information from ECoGs, EMGs, brain temperature, and locomotor activity. Detailed classification criteria are stated in the text.

Manipulation of Epileptiform Electrocorticograms ECoGs and Sleep in Rats and Mice by Acupuncture

This paper demonstrates the performance of acupuncture, epilepsy models, and the analysis of sleep in rodents. The acupuncture procedure and the identification of acupoints are described. Pilocarpine or pentylenetetrazol (PTZ) is used to induce epilepsy. Electrocorticogram (ECoG), electromyogram (EMG), brain temperature, and locomotor activity recordings are employed for sleep analysis.

Ancient Chinese literature has documented that acupuncture possesses efficient therapeutic effects on epilepsy and insomnia. There is, however, little ...

Assessing Spatial Memory Impairment in a Mouse Model of Traumatic Brain Injury Using a Radial Water Tread Maze

Despite the recent increase in use of mouse models in scientific research, researchers continue to use cognitive tasks that were originally designed and validated for rat use. The Radial Water Tread (RWT) maze test of spatial memory (designed specifically for mice and requiring no swimming) has been shown previously to successfully distinguish between controlled cortical impact-induced TBI mice and sham controls. Here, a detailed protocol for this task is presented. The RWT maze capitalizes on the natural tendency of mice to avoid open areas in favor of hugging the sides of an apparatus (thigmotaxis). The walls of the maze are lined with nine escape holes placed above the floor of the apparatus, and mice are trained to use visual cues to locate the escape hole that leads out of the maze. The maze is filled with an inch of cold water, sufficient to motivate escape but not deep enough to require that the mouse swim. The acquisition period takes only four training days, with a test of memory retention on day five and a long-term memory test on day 12. The results reported here suggest that the RWT maze is a feasible alternative to rat-validated, swimming-based cognitive tests in the assessment of spatial memory deficits in mouse models of TBI.

Here we present a protocol for a mouse-specific test of cognition that does not require swimming. This test can be used to successfully distinguish controlled cortical impact-induced traumatic brain injury mice from sham controls.

Despite the recent increase in use of mouse models in scientific research, researchers continue to use cognitive tasks that were originally designed and ...

Detecting Behavioral Deficits in Rats After Traumatic Brain Injury

With the increasing incidence of traumatic brain injury (TBI) in both civilian and military populations, TBI is now considered a chronic disease; however, few studies have investigated the long-term effects of injury in rodent models of TBI. Shown here are behavioral measures that are well-established in TBI research for times early after injury, such as two weeks, until two months. Some of these methods have previously been used at later times after injury, up to one year, but by very few laboratories. The methods demonstrated here are a short neurological assessment to test reflexes, a Beam-Balance to test balance, a Beam-Walk to test balance and motor coordination, and a working memory version of the Morris water maze that can be sensitive to deficits in reference memory. Male rats were handled and pre-trained to neurological, balance, and motor coordination tests prior to receiving parasagittal fluid percussion injury (FPI) or sham injury. Rats can be tested on the short neurological assessment (neuroscore), the beam-balance, and the Beam-Walk multiple times, while testing on the water maze can only be done once. This difference is because rats can remember the task, thus confounding the results if repeated testing is attempted in the same animal. When testing from one to three days after injury, significant differences are detected in all three non-cognitive tasks. However, differences in the Beam-Walk task were not detectable at later time points (after 3 months). Deficits were detected at 3 months in the Beam-Balance and at 6 months in the neuroscore. Deficits in working memory were detected out to 12 months after injury, and a deficit in a reference memory first appeared at 12 months. Thus, standard behavioral tests can be useful measures of persistent behavioral deficits after FPI.

The goal of the behavioral tests presented here is to detect functional deficits in rats after traumatic brain injury. Four specific tests are presented that detect deficits in behaviors to reflect the damage to specific brain areas at times extending to one year after injury.

With the increasing incidence of traumatic brain injury (TBI) in both civilian and military populations, TBI is now considered a chronic disease; however, ...

Using Laser Doppler Imaging and Monitoring to Analyze Spinal Cord Microcirculation in Rat

Laser Doppler flowmetry (LDF) is a noninvasive method for blood flow (BF) measurement, which makes it preferable for measuring microcirculatory alterations of the spinal cord. In this article, our goal was to use both Laser Doppler imaging and monitoring to analyze the change of BF after spinal cord injury. Both the laser Doppler image scanner and the probe/monitor were being employed to obtain each readout. The data of LDPI provided a local distribution of BF, which gave an overview of perfusion around the injury site and made it accessible for comparative analysis of BF among different locations. By intensely measuring the probing area over a period of time, a combined probe was used to simultaneously measure the BF and oxygen saturation of the spinal cord, showing overall spinal cord perfusion and oxygen supply. LDF itself has a few limitations, such as relative flux, sensitivity to movement, and biological zero signal. However, the technology has been applied in clinical and experimental study due to its simple setup and rapid measurement of BF.

Here we present a combination of laser Doppler perfusion imaging (LDPI) and laser Doppler perfusion monitoring (LDPM) to measure spinal cord local blood flows and oxygen saturation (SO2), as well as a standardized procedure for introducing spinal cord trauma on rat.

Laser Doppler flowmetry (LDF) is a noninvasive method for blood flow (BF) measurement, which makes it preferable for measuring microcirculatory ...

Use of a Video Scoring Anchor for Rapid Serial Assessment of Social Communication in Toddlers

Reciprocal social behavior (RSB), an early-emerging capacity to engage in social contingency&#8212;which is foundational for both social learning and social competency&#8212;is hypothesized to be disrupted in autism spectrum disorder (ASD). The ability to quantify the full range of RSB during the toddler period, when core symptoms of ASD often arise, is pivotal for evaluating early risk for ASD, characterizing social development, and tracking response to early interventions. However, important parameters of variation in RSB&#8212;especially prior to the development of verbal language&#8212;can be nuanced and difficult to characterize using questionnaire-based methods. To address this challenge, we developed a system for measuring quantitative variation in RSB in toddlers (ages 18 - 30 months) that incorporated not only standard questionnaire data from caregivers but also a novel set of video-referenced items, through which a respondent compares the behavior of a subject to that observed in a short video of a young child manifesting a highly competent level of social communication. Testing of this measure in a general population sample of twins confirmed that both the video-referenced items and the RSB Total Score (video-referenced items plus non-video-referenced items) displayed unimodal, continuous distributions, strong internal consistency, marked preservation of individual differences, and extremely high heritability. In addition, video-referenced items were particularly sensitive to quantifying incremental changes in social communication, a major element of RSB, over the course of early childhood development. Scores on the vrRSB clearly differentiated children with and without ASD and these data comprise an initial validation of this promising method for quantifying early RSB&#8212;cross-sectionally, over time, and as a function of early intervention.

Here we present a protocol for administration of the video-referenced rating of reciprocal social behavior (vrRSB) using a paper or online version. This survey quantifies RSB, a prerequisite for social competence, in toddlers through &#34;video-referenced&#34; items whereby a subject is compared to a reference child shown in a three-minute video.

Reciprocal social behavior (RSB), an early-emerging capacity to engage in social contingency&#8212;which is foundational for both social learning and ...