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A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction

16.0K Views

00:05 min

August 27th, 2019

DOI :

10.3791/59656-v

August 27th, 2019


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DARTS Assay

Chapters in this video

0:04

Title

0:47

Collect and Lyse Cells

1:59

Incubate Protein Lysates with the Small Molecule and Perform Proteolysis

3:28

Western Blot Analysis

3:59

Results: Rapamycin Inhibition

5:03

Conclusion

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