Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells

7.4K Views

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10:18 min

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June 13th, 2019

DOI :

10.3791/59815-v

June 13th, 2019

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Vinciane Saint-Criq¹, Iram J. Haq²^,³, Aaron I. Gardner², James P. Garnett²^,⁴, Christopher Ward¹^,², Malcolm Brodlie²^,³, Michael A. Gray¹

¹Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, ²Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, ³Paediatric Respiratory Medicine, Great North Children's Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust, ⁴Boehringer Ingelheim Pharma GmbH & Co

Summary

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Real time Measurement

Airway Surface Liquid

ASL PH

Primary Human Airway Epithelial Cells

Ion Channels

Extracellular PH Regulation

Chronic Airway Diseases

Cystic Fibrosis

Asthma

COPD

Cell Culture Incubator

Bicarbonate Krebs Buffer

Chapters in this video

0:04

Title

1:09

Primary Human Airway Epithelial Cell (hAEC) Preparation

1:53

Background Measurement

4:43

Kinetics Measurement

5:45

In Situ pH Calibration

6:28

Data Analysis

7:58

Results: Representative pH Calibration in Primary hAECs In Situ

9:34

Conclusion

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