Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
7.4K Views
•
10:18 min
•
June 13th, 2019
DOI :
June 13th, 2019
•Vinciane Saint-Criq1, Iram J. Haq2,3, Aaron I. Gardner2, James P. Garnett2,4, Christopher Ward1,2, Malcolm Brodlie2,3, Michael A. Gray1
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, 3Paediatric Respiratory Medicine, Great North Children's Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co
Chapters in this video
0:04
Title
1:09
Primary Human Airway Epithelial Cell (hAEC) Preparation
1:53
Background Measurement
4:43
Kinetics Measurement
5:45
In Situ pH Calibration
6:28
Data Analysis
7:58
Results: Representative pH Calibration in Primary hAECs In Situ
9:34
Conclusion
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