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Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

6.0K Views

08:31 min

November 15th, 2019

November 15th, 2019

6,030 Views

0:04

Title

1:30

Cell Seeding and pEB3-tdTomato Expression

2:37

Synchronization and Live-cell Imaging

3:56

Analysis of Microtubule Dynamics

6:29

Results: Analysis of Microtubule Dynamics in HeLa Cells Synchronized in Prometaphase

7:50

Conclusion

Transcript

What we present here is a robust and simple method to analyze microtubule dynamics using live-cell spinning disk confocal imaging in cells synchronized in prometaphase, and images are afterwards analyzed on a MATLAB-based platform. The use of red-

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Here we present a robust and detailed method of microtubule dynamics analysis in cells synchronized in prometaphase using live-cell spinning disk confocal microscopy and MATLAB-based image processing.

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