Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
7.6K Views
•
10:17 min
•
January 14th, 2020
DOI :
January 14th, 2020
•Chapters in this video
0:04
Title
0:47
TG1 Cell and Negative Selection Bead Preparation
2:15
Positive Selection Bead Preparation
3:33
Phage-displayed Nanobody Elution and Input/Output Titrations and Infection
5:15
Single Clone Isolation
6:22
Anchor Binder Validation
8:13
Results: Representative Anchor and Dimerization Binder Characterization
9:39
Conclusion
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