Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

7.6K Views

12:19 min

February 12th, 2020

DOI :

10.3791/60824-v

February 12th, 2020


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C2C12 Myoblast

Chapters in this video

0:04

Introduction

0:55

C2C12 Transfection

2:35

Transfected C2C12 Selection

3:15

C2C12 Differentiation

3:59

Phenotypic Analysis

6:10

Western Blot Analysis Sample Preparation

9:37

Results: Representative C2C12 Selection, Differentiation, and Knockdown Validation

11:19

Conclusion

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