Proximal Differentiation to Generate Mature Airway Organoids
7:42
Results: Deriving Human Lung Organoids and Proximal Differentiation to Generate 3D and 2D Airway Organoids
9:38
Conclusion
Transcript
We are introducing a method to establish the organoid culture of human airway epithelium in culture plates. We derive human lung organoid directly from primary lung tissues with high efficiency. The derived human lung organoid are stably expanded
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The protocol presents a method to derive human lung organoids from primary lung tissues, expand the lung organoids and induce proximal differentiation to generate 3D and 2D airway organoids that faithfully phenocopy the human airway epithelium.