Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

4.6K Views

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11:40 min

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January 21st, 2021

DOI :

10.3791/61599-v

January 21st, 2021

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Gabriella Shull*¹^,², Christiane Haffner*³, Wieland B. Huttner³, Elena Taverna³^,⁴, Suhasa B. Kodandaramaiah¹^,⁵^,⁶

¹Department of Biomedical Engineering, University of Minnesota, ²Department of Biomedical Engineering, Duke University, ³Max Planck Institute of Molecular Cell Biology and Genetics, ⁴Max Planck Institute for Evolutionary Anthropology, ⁵Department of Mechanical Engineering, University of Minnesota, ⁶Graduate Program in Neuroscience, University of Minnesota

Summary

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Neural Stem Cells

Brain Slices

Robotic Microinjection

Tissue Formation

Visual Demonstration

Microinjection Protocol

Agarose Embedding

Tyrode s Solution

Fluorescent Tracer

Organotypic Tissue Slices

Mouse Embryos

Dissection Technique

Embryonic Brain Manipulation

Microinjection Solution Preparation

Chapters in this video

0:05

Introduction

1:02

Tissue Slice Preparation

4:04

Microinjection

7:05

Tissue Culture and Tissue Slice Processing for Immunofluorescence

9:39

Results: Robotic Microinjection into Apical Progenitors and Neurons

10:57

Conclusion

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