Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

6.6K Views

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07:02 min

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November 3rd, 2020

DOI :

10.3791/61605-v

November 3rd, 2020

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Lisa Johann¹^,², Oleksandra Chabanovska¹^,², Cajetan Immanuel Lang³, Robert David¹^,², Heiko Lemcke¹^,²

¹Department of Cardiac Surgery, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Rostock University Medical Center, ²Faculty of Interdisciplinary Research, Department Life, Light & Matter, University Rostock, ³Department of Cardiology, Rostock University Medical Center

Summary

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Actinin Network

Human IPSC derived Cardiomyocytes

Single Molecule Localization Microscopy

Sarcomere Maturation

Super resolution Imaging

Image Acquisition Parameters

Chapters in this video

0:04

Introduction

0:27

Photoactivated Localization Microscopy (PALM) Image Acquisition Setup

1:27

PALM Image Acquisition

1:50

PALM Data Reconstruction

2:39

Reconstructed PALM Image Post Processing

3:34

Sarcomere Filament Analysis

4:41

Results: Representative Human Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocyte alpha-Actinin Network Analysis

6:23

Conclusion

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