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Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

DOI :

10.3791/62010-v

July 27th, 2021

July 27th, 2021

3,533 Views

1Department of Biochemistry, Emory University School of Medicine

For in-depth mechanistic analysis of the respiratory syncytial virus (RSV) RNA synthesis, we report a protocol of utilizing the chaperone phosphoprotein (P) for coexpression of the RNA-free nucleoprotein (N0) for subsequent in vitro assembly of the virus-specific nucleocapsids (NCs).

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